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Simple Plex Human Growth Hormone Cartridge

R&D Systems | Catalog # SPCKB-PS-000492

Simple Plex Human Growth Hormone assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.
R&D Systems

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Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Serum (5 µL), EDTA Plasma (5 µL), Heparin Plasma (5 µL)

Sensitivity

0.67 pg/mL

Assay Range

1.74-16610 pg/mL (Serum, EDTA Plasma, Heparin Plasma)

Simple Plex Human Growth Hormone Cartridge Summary

Simple Plex assay is for the detection of human Growth Hormone (GH) in serum and plasma (EDTA/Heparin) in Sample Diluent SD13.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all Growth Hormone Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
Learn More

Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human GH.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one GH assay.

Inter-Assay Precision (Precision between assays) Replicates of each GH control were tested in multiple assays performed by at least three technicians using two lots of reagents for human GH.

EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 32 32
Mean (pg/mL) 93.8 4195 87.6 4425
Standard Deviation 5.86 334 6.63 397
CV% 6.3 8.0 7.6 9.0

Recovery for Simple Plex Human Growth Hormone Cartridge

Recovery was not necessary as human GH shows 5 points of natural linearity.

Linearity

Samples containing and/or spiked with high concentrations of human GH were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Simple Plex Human GH Assay Linearity

Scientific Data Images for Simple Plex Human Growth Hormone Cartridge

Simple Plex Human GH Assay Standard Curve

Simple Plex Human GH Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human GH Assay. Assay range is 1.74-16,610 pg/mL.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Growth Hormone

GH (Growth Hormone; Somatotropin) is a polypeptide hormone that regulates somatic growth and metabolism through interactions with Growth Hormone R/GHR. GH is released from the anterior pituitary in response to GH-releasing Hormone (GHRH) and Ghrelin, while its secretion is suppressed by Somatostatin. GH levels may also be regulated by Thyrotropin-releasing Hormone, Leptin, Galanin, Neuropeptide Y, and Cortisol. GH can circulate in complex with a shed soluble form of GHR known as GHBP. In addition to promoting bone and muscle mass, GH exerts immunomodulatory effects and induces lipolysis, lactation, amino acid uptake, and protein synthesis. Overproduction of GH can lead to acromegaly, a disorder characterized by enlargement of the hands, feet, and facial features, as well as enhanced risk of cardiovascular disease, respiratory disease, and malignancy. GH deficiency in adults may lead to increases in cardiovascular risk, body fat, and insulin sensitivity, as well as decreased muscle mass and bone density.

Alternate Names

GH-N, GH1, GHB5, GHN, IGHD1A, IGHD2, Somatotropin

Entrez Gene IDs

2688 (Human)

Gene Symbol

GH1

Additional Growth Hormone Products

Product Documents for Simple Plex Human Growth Hormone Cartridge

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Certificate of Analysis

To download a Certificate of Analysis, please enter your Simple Plex catalog number and lot number in the search boxes below.

Product Specific Notices for Simple Plex Human Growth Hormone Cartridge

For research use only

Citations for Simple Plex Human Growth Hormone Cartridge

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