Simple Plex Human HGFR/c-MET Cartridge

Catalog #: SPCKB-PS-000325 Datasheet / COA / SDS

Simple Plex Human HGFR/c-MET assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer

Catalog #	Availability	Size / Price	Qty
SPCKB-PS-000325
SPCKA-PS-000325
SPCKC-PS-000325

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898327: Simple Plex Control for Human HGFR/c-MET

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Human HGFR/c-MET Cartridge Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Serum (10 uL), EDTA Plasma (500 nL), Heparin Plasma (10 uL)

Sensitivity

1.9 pg/mL

Assay Range

4.2 - 100,000 pg/mL (Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant human HGF R.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay is for the detection of human Hepatocyte Growth Factor Receptor (HGF R) in serum and plasma (EDTA/Heparin) in Sample Diluent SD13.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one HGF R assay

Inter-Assay Precision (Precision between assays) Replicates of each HGF R control were tested in multiple assays performed by at least three technicians using two lots of reagents for human HGF R

Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	32	33
Mean (pg/mL)	299	13605	272	13483
Standard Deviation	21.2	849	23.7	1263
CV%	7.2	6.2	8.7	9.4

Recovery

Recovery was not necessary as human HGF R shows 5 points of natural linearity.

Linearity

Samples containing and/or spiked with high concentrations of human HGF R were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data

N/A

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Simple Plex Human HGF R Assay Standard Curve Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human HGF R Assay. Assay range is 4.20-100,000 pg/mL.

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: HGFR/c-MET

HGF receptor, a product of the proto-oncogene c-met, is a heterodimeric transmembrane glycoprotein that is a receptor-type tyrosine kinase. c-MET is synthesized as a single-chain precursor (pr170) which undergoes post-translational glycosylation and proteolytic cleavage to give rise to the heterodimeric mature form.

Long Name:

Hepatocyte Growth Factor Receptor

Entrez Gene IDs:

4233 (Human); 17295 (Mouse)

Alternate Names:

AUTS9; cMET; c-MET; EC 2.7.10; EC 2.7.10.1; hepatocyte growth factor receptor; HGF R; HGF receptor; HGF/SF receptor; HGFR; Met (c-Met); met proto-oncogene (hepatocyte growth factor receptor); met proto-oncogene tyrosine kinase; MET; oncogene MET; Proto-oncogene c-Met; RCCP2; Scatter factor receptor; SF receptor; Tyrosine-protein kinase Met