Simple Plex Human HGFR/c-MET Cartridge

Name: Simple Plex Human HGFR/c-MET Cartridge
Brand: R&D Systems
SKU: SPCKB-PS-000325
Price: 450 USD
Availability: InStock

R&D Systems | Catalog # SPCKB-PS-000325

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Simple Plex Human HGFR/c-MET assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.

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Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and up to 8 analytes per cartridge. Customize this cartridge.

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Compatible Instruments
Product Specifications
Precision
Recovery
Linearity
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Serum (10 µL), EDTA Plasma (500 nL), Heparin Plasma (10 µL)

Sensitivity

1.9 pg/mL

Assay Range

4.2-100000 pg/mL (Serum, EDTA Plasma, Heparin Plasma)

Simple Plex Human HGFR/c-MET Cartridge Summary

Simple Plex assay is for the detection of human Hepatocyte Growth Factor Receptor (HGF R) in serum and plasma (EDTA/Heparin) in Sample Diluent SD13.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

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Compatible Instruments

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.

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Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human HGF R.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one HGF R assay.

Inter-Assay Precision (Precision between assays) Replicates of each HGF R control were tested in multiple assays performed by at least three technicians using two lots of reagents for human HGF R.

EDTA Plasma, Heparin Plasma, Serum

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	32	33
Mean (pg/mL)	299	13,605	272	13,483
Standard Deviation	21.2	849	23.7	1263
CV%	7.2	6.2	8.7	9.4

Recovery for Simple Plex Human HGFR/c-MET Cartridge

Recovery was not necessary as human HGF R shows 5 points of natural linearity.

Linearity

Samples containing and/or spiked with high concentrations of human HGF R were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data Images for Simple Plex Human HGFR/c-MET Cartridge

Simple Plex Human HGF R Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human HGF R Assay. Assay range is 4.20-100,000 pg/mL.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: HGFR/c-MET

HGFR (Hepatocyte Growth Factor Receptor), also known as Met, is a receptor tyrosine kinase that plays a central role in epithelial morphogenesis and cancer development. In the absence of ligand, HGFR forms noncovalent complexes with a variety of membrane proteins including CD44v6, CD151, EGFR, Fas, Integrin alpha 6 beta 4, Plexins B1, 2, 3, and MSPR/Ron. Ligation of one complex component triggers activation of the other, followed by cooperative signaling effects. Formation of some of these heteromeric complexes is a requirement for epithelial cell morphogenesis and tumor cell invasion. Paracrine induction of epithelial cell scattering and branching tubulogenesis results from the stimulation of HGFR on undifferentiated epithelium by HGF released from neighboring mesenchymal cells.