Simple Plex Human IL-1 alpha Cartridge

Catalog #: SPCKB-PS-000503 Datasheet / COA / SDS
Simple Plex Human IL-1 alpha/IL-1F1 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer
Catalog # Availability Size / Price Qty
SPCKB-PS-000503
SPCKA-PS-000503
SPCKC-PS-000503
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Human IL-1 alpha  Simple Plex Assay
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Simple Plex Human IL-1 alpha Cartridge Summary

Sensitivity
0.831 pg/mL
Assay Range
0.8 - 1,670 pg/mL (Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human IL1 alpha.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of human IL-1 alpha in serum and plasma (EDTA/Heparin)

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 16 16
Mean (pg/mL) 12.4 640 12.2 577
Standard Deviation 0.41 27.9 0.448 47.8
CV% 3.3 4.4 3.7 8.3

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type Average % Recovery Range %
EDTA Plasma (n=4) 102 97-108
Heparin Plasma (n=4) 102 99-105
Serum (n=4) 104 97-112

Linearity

Samples containing and/or spiked with high concentrations of human IL-1 alpha were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
Human IL-1 alpha  Simple Plex Assay Linearity

Scientific Data

Human IL-1 alpha Simple Plex Assay Standard Curve

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Preparation and Storage

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-1 alpha/IL-1F1

IL-1 alpha (Interleukin-1 alpha) is a cytokine that is induced during microbial exposure and promotes the release of multiple inflammatory mediators and acute phase proteins. It also supports hematopoietic stem cell expansion, promotes neutrophil mobilization from the bone marrow, and promotes osteoclast formation. IL-1 alpha signals through a receptor complex composed of IL-1 RI and IL-1 RAcP. It also binds the decoy receptor IL-1 RII which associates with IL-1 RAcP but does not transduce signaling.

Long Name:
Interleukin 1 alpha
Entrez Gene IDs:
3552 (Human); 16175 (Mouse); 24493 (Rat); 397094 (Porcine); 101926546 (Cynomolgus Monkey)
Alternate Names:
BAF; Hematopoietin-1; IL1 alpha; IL-1 alpha; IL1; IL1A; IL-1A; IL1-ALPHA; IL1F1; IL-1F1; IL1F1hematopoietin-1; interleukin 1, alpha; interleukin-1 alpha; LAF; LEM; preinterleukin 1 alpha; pro-interleukin-1-alpha

FAQs

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