Simple Plex Human IL-1 alpha Cartridge

R&D Systems | Catalog # SPCKB-PS-000503

Simple Plex Human IL-1 alpha/IL-1F1 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.
R&D Systems

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Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 µL), Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL)

Sensitivity

0.314 pg/mL

Assay Range

0.49-1880 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Simple Plex Human IL-1 alpha Cartridge Summary

Simple Plex assay is for the detection of human Interleukin 1 alpha (IL-1 alpha ) in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin) in Sample Diluent SD13.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all IL-1 alpha/IL-1F1 Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
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Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human IL1 alpha.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one IL-1 alpha assay.

Inter-Assay Precision (Precision between assays) Replicates of each IL-1 alpha control were tested in multiple assays performed by at least three technicians using two lots of reagents for human IL-1 alpha.

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 15 15
Mean (pg/mL) 12.4 640 12.2 577
Standard Deviation 0.410 27.9 0.448 47.8
CV% 3.3 4.4 3.7 8.3

Recovery for Simple Plex Human IL-1 alpha Cartridge

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=4) 121 106-131
EDTA Plasma (n=4) 102 97-108
Heparin Plasma (n=4) 102 99-105
Serum (n=4) 104 97-112

Linearity

Samples containing and/or spiked with high concentrations of human IL-1 alpha were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Simple Plex Human IL-1 alpha Assay Linearity

Scientific Data Images for Simple Plex Human IL-1 alpha Cartridge

Simple Plex Human IL-1 alpha Assay Standard Curve

Simple Plex Human IL-1 alpha Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human IL-1 alpha Assay. Assay range is 0.49-1800 pg/mL.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-1 alpha/IL-1F1

IL-1 alpha (Interleukin-1 alpha) is a cytokine that is induced during microbial exposure and promotes the release of multiple inflammatory mediators and acute phase proteins. It also supports hematopoietic stem cell expansion, promotes neutrophil mobilization from the bone marrow, and promotes osteoclast formation. IL-1 alpha signals through a receptor complex composed of IL-1 RI and IL-1 RAcP. It also binds the decoy receptor IL-1 RII which associates with IL-1 RAcP but does not transduce signalling.

 

Long Name

Interleukin 1 alpha

Alternate Names

BAF, IL-1A, IL-1F1, IL1, IL1 alpha, IL1A, IL1F1, LAF, LEM

Entrez Gene IDs

3552 (Human); 16175 (Mouse); 24493 (Rat); 397094 (Porcine); 101926546 (Cynomolgus Monkey)

Gene Symbol

IL1A

Additional IL-1 alpha/IL-1F1 Products

Product Documents for Simple Plex Human IL-1 alpha Cartridge

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Certificate of Analysis

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Product Specific Notices for Simple Plex Human IL-1 alpha Cartridge

For research use only

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Associated Pathways

IL-1 Family Signaling Pathways IL-1 Family Signaling Pathway Thumbnail