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Simple Plex Human IL-18 Cartridge

Name: Simple Plex Human IL-18 Cartridge
Brand: R&D Systems
SKU: SPCKB-PS-000501
Price: 885 USD
Availability: InStock

R&D Systems | Catalog # SPCKB-PS-000501

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Product Documents

Simple Plex Human IL-18/IL-1F4 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer. Sample type indicated with PS (Plasma/Serum).

Compatible Instruments
Product Specifications
Precision
Recovery
Linearity
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (25 µL), Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL), Urine (10 µL)

Sensitivity

0.2 pg/mL

Assay Range

2.4-3660 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)

Simple Plex Human IL-18 Cartridge Summary

Simple Plex assay is for the detection of human Interleukin 18 (IL-18) in cell culture supernatant (CCS), serum, plasma (EDTA/Heparin), and urine in Sample Diluent SD13.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all IL-18/IL-1F4 Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.

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Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human IL-18.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one IL-18 assay.

Inter-Assay Precision (Precision between assays) Replicates of each IL-18 control were tested in multiple assays performed by at least three technicians using two lots of reagents for human IL-18.

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum, Urine

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	15	14
Mean (pg/mL)	10.5	569	10.3	587
Standard Deviation	0.524	18.7	0.882	58.4
CV%	5.2	3.3	8.6	10.0

Recovery for Simple Plex Human IL-18 Cartridge

Recovery for the human IL-18 assays was evaluated at three different spiked concentrations of IL-18 protein.

Sample Type	Average % Recovery	Range %
Urine (n=5)	120	114-128

Linearity

Samples containing and/or spiked with high concentrations of human IL-18 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Images for Simple Plex Human IL-18 Cartridge

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human IL-18 Assay. Assay range is 2.40-3660 pg/mL.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-18/IL-1F4

IL-18 (Interleukin-18) is secreted by macrophages, dendritic cells, and epithelial cells. Circulating mature IL-18 is sequestered by soluble IL-18 binding proteins (IL-18 BP) that inhibit IL-18 bioactivity. IL-18 signals through a receptor complex that contains IL-18 R alpha IL-18 R beta. It synergizes with other cytokines to activate NK, Th1, and Th17 cells and to increase the production of IFN-gamma. It can also promote Th2 cytokine release which reduces the effectiveness of antiviral responses. Increased levels of active IL-18 contribute to the severity of autoimmunity and hypertension, while deficiency of IL-18 results in symptoms of metabolic syndrome. In cancer, IL-18 stimulates Th1 and NK cells to target tumor cells, but it can also promote angiogenesis, metastasis, and tumor cell immune evasion.