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Simple Plex Human IL-8 Cartridge

R&D Systems | Catalog # SPCKB-PS-000230

Simple Plex Human IL-8/CXCL8 assay kit contains cartridge, sample diluent SD13, and wash buffer. Sample type indicated with PS (Plasma/Serum).
R&D Systems

Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (25 µL), Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL), Urine (25 µL), Cerebrospinal Fluid (25 µL)

Sensitivity

0.08 pg/mL

Assay Range

0.19-1804 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine, Cerebrospinal Fluid)

Simple Plex Human IL-8 Cartridge Summary

Simple Plex assay for the detection of human IL-8/CXCL8 (IL-8) in cell culture supernatant (CCS), serum, plasma (EDTA/Heparin), urine, and cerebrospinal fluid (CSF) in Sample Diluent SD13.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all IL-8/CXCL8 Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
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Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human IL-8.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one IL-8 assay.

Inter-Assay Precision (Precision between assays) Replicates of each IL-8 control were tested in multiple assays performed by at least three technicians using two lots of reagents for human IL-8.

Cell Culture Supernates, Cerebrospinal Fluid, EDTA Plasma, Heparin Plasma, Serum, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 10 10
Mean (pg/mL) 28.7 1362 27.3 1463
Standard Deviation 1.41 89.8 1.41 125.0
CV% 4.9 6.6 5.2 8.6

Recovery for Simple Plex Human IL-8 Cartridge

Recovery for the human IL-8 assays was evaluated at three different spiked concentrations of IL-8 protein.

Sample Type Average % Recovery Range %
EDTA Plasma (n=5) 89 75-102
Heparin Plasma (n=5) 95 88-102
Serum (n=3) 92 88-97

Linearity

Samples containing and/or spiked with high concentrations of human IL-8 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Simple Plex Human IL-8 Assay Linearity

Scientific Data Images for Simple Plex Human IL-8 Cartridge

Simple Plex Human IL-8 Assay Standard Curve

Simple Plex Human IL-8 Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human IL-8 Assay. Assay range is 0.19-1804 pg/mL

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-8/CXCL8

Interleukin-8 (IL-8), also known as CXCL8, is a chemokine that is upregulated at sites of inflammation where it promotes neutrophil infiltration and activation. IL-8 can form homodimers and heterodimers with CXCL4/PF4. Its bioactivity is regulated by proteolytic truncations, citrullination, and the decoy receptor DARC. IL-8 signals through CXCR1/IL-8 RA, which is also used by CXCL6, and through CXCR2/IL-8 RB, which is used by multiple CXC chemokines. IL-8 also binds to Serpin A1/alpha-1 Antitrypsin which prevents IL-8 interaction with CXCR1.

Long Name

Interleukin 8

Alternate Names

CXCL8, GCP1, IL8, LAI, LECT, LUCT, LYNAP, MDNCF, MONAP, NAF, NAP1, NCF, TCF, TSG1

Entrez Gene IDs

3576 (Human); 396880 (Porcine); 403850 (Canine); 493836 (Feline)

Gene Symbol

CXCL8

Additional IL-8/CXCL8 Products

Product Documents for Simple Plex Human IL-8 Cartridge

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Certificate of Analysis

To download a Certificate of Analysis, please enter your Simple Plex catalog number and lot number in the search boxes below.

Product Specific Notices for Simple Plex Human IL-8 Cartridge

For research use only

Citations for Simple Plex Human IL-8 Cartridge

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FAQs for Simple Plex Human IL-8 Cartridge

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  • Q: Are mouse or rat CXCL8/IL-8 products offered?

    A: Mouse and rat do not have a gene which encodes CXCL8/IL-8. In mouse, the functional homologs to human CXCL8/IL-8 are CXCL1/KC and CXCL2/MIP-2. In rat, the functional homolog to CXCL8/human IL-8 is CXCL3/CINC-2.

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