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Simple Plex Human IL-8 Cartridge

R&D Systems | Catalog # SPCKB-PS-000230

Simple Plex Human IL-8/CXCL8 assay kit contains cartridge, sample diluent SD13, and wash buffer. Sample type indicated with PS (Plasma/Serum).
R&D Systems

Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (25 µL), Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL), Urine (25 µL), Cerebrospinal Fluid (25 µL)

Sensitivity

0.08 pg/mL

Assay Range

0.19-1804 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine, Cerebrospinal Fluid)

Simple Plex Human IL-8 Cartridge Summary

Simple Plex assay for the detection of human IL-8/CXCL8 (IL-8) in cell culture supernatant (CCS), serum, plasma (EDTA/Heparin), urine, and cerebrospinal fluid (CSF).

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all CD163 Simple Plex »

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Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human IL-8.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay.

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents.

Cell Culture Supernates, Cerebrospinal Fluid, EDTA Plasma, Heparin Plasma, Serum, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 10 10
Mean (pg/mL) 28.7 1362 27.3 1463
Standard Deviation 1.41 89.8 1.41 125.0
CV% 4.9 6.6 5.2 8.6

Recovery for Simple Plex Human IL-8 Cartridge

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type Average % Recovery Range %
EDTA Plasma (n=5) 89 75-102
Heparin Plasma (n=5) 95 88-102
Serum (n=3) 92 88-97

Linearity

Samples containing and/or spiked with high concentrations of human IL-8 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Human IL-8 Simple Plex Assay Linearity

Scientific Data Images for Simple Plex Human IL-8 Cartridge

Human IL-8 Simple Plex Assay Standard Curve

Human IL-8 Ella Assay Standard Curve

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CD163

CD163, also known as M130 and  p155, is a transmembrane scavenger receptor that is expressed on monocytes and macrophages and is inducible by immunosuppressant glucocorticoids and IL-10. A soluble form is shed from the cell surface by TACE or neutrophil elastase mediated cleavage in response to oxidative stress, Prostaglandin F2a stimulation, or the activation of Fc gamma receptors, TLR1, 2, 5, or 6. CD163 mediates monocyte binding to bacteria, leading to the release of inflammatory cytokines. It is essential for the circulatory clearance of hemoglobin-haptoglobin (Hb-Hp) complexes as well as free hemoglobin. It can also mediate monocyte-erythroblast adhesion and promote erythroblast expansion. CD163 binds and internalizes the cytokine TWEAK, and the ratio of soluble CD163 to TWEAK in the plasma is elevated during atherosclerosis.

Alternate Names

CD163, GHI/61, HbSR, M130, RM3/1

Entrez Gene IDs

9332 (Human); 93671 (Mouse); 312701 (Rat)

Gene Symbol

CD163

Additional CD163 Products

Product Documents for Simple Plex Human IL-8 Cartridge

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Certificate of Analysis

To download a Certificate of Analysis, please enter your Simple Plex catalog number and lot number in the search boxes below.

Product Specific Notices for Simple Plex Human IL-8 Cartridge

For research use only

Citations for Simple Plex Human IL-8 Cartridge

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FAQs for Simple Plex Human IL-8 Cartridge

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  • Q: Are mouse or rat CXCL8/IL-8 products offered?

    A: Mouse and rat do not have a gene which encodes CXCL8/IL-8. In mouse, the functional homologs to human CXCL8/IL-8 are CXCL1/KC and CXCL2/MIP-2. In rat, the functional homolog to CXCL8/human IL-8 is CXCL3/CINC-2.

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