Simple Plex Human M-CSF Cartridge
R&D Systems | Catalog # SPCKB-PS-000403
Customize This Cartridge
Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and up to 8 analytes per cartridge. Customize this cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human M-CSF Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Species
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one M-CSF assay.
Inter-Assay Precision (Precision between assays) Replicates of each M-CSF control were tested in multiple assays performed by at least three technicians using two lots of reagents for human M-CSF.
Bronchoalveolar Lavage Fluid, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 16 | 17 |
| Mean (pg/mL) | 83.9 | 3769 | 78.0 | 3557 |
| Standard Deviation | 7.58 | 325 | 6.52 | 312 |
| CV% | 9.0 | 8.6 | 8.4 | 8.8 |
Recovery for Simple Plex Human M-CSF Cartridge
Recovery for the human M-CSF assays was evaluated at three different spiked concentrations of M-CSF protein.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| EDTA Plasma (n=4) | 91 | 82-101 |
| Heparin Plasma (n=4) | 90 | 84-96 |
| Serum (n=4) | 90 | 88-95 |
Linearity
Samples containing and/or spiked with high concentrations of human M-CSF were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
Scientific Data Images for Simple Plex Human M-CSF Cartridge
Simple Plex Human M-CSF Assay Standard Curve
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human M-CSF Assay. Assay range is 3.11-11,880 pg/mL.Preparation and Storage
Shipping
Stability & Storage
Background: M-CSF
M-CSF (CSF-1) is the primary regulator of macrophage survival, proliferation and differentiation. It is essential for macrophage-related functions such as bone resorption, vascular development, and innate immunity. It signals through M-CSF R on monocytes, tissue macrophages, and osteoblasts. Both M-CSF and its receptor can be expressed by tumor cells, allowing M-CSF to act as an autocrine growth factor for cancer cells. Full length M-CSF is expressed as a transmembrane dimer, but various additional forms can be generated by alternative splicing, differential glycosylation, chondroitin sulfate proteoglycan modification, and proteolytic shedding.
Long Name
Alternate Names
Gene Symbol
Additional M-CSF Products
Product Documents for Simple Plex Human M-CSF Cartridge
Certificate of Analysis
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Product Specific Notices for Simple Plex Human M-CSF Cartridge
For research use only
Citations for Simple Plex Human M-CSF Cartridge
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