Simple Plex Human Myeloperoxidase/MPO Cartridge

R&D Systems | Catalog # SPCKB-PS-000517

Simple Plex Human Myeloperoxidase/MPO assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.
R&D Systems

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Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 µL), Serum (10 µL), Platelet-poor EDTA Plasma (10 µL), Platelet-poor Heparin Plasma (10 µL), Urine (10 µL), Cerebrospinal Fluid (10 µL)

Sensitivity

0.598 pg/mL

Assay Range

3.14-30000 pg/mL (Cell Culture Supernates, Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Urine, Cerebrospinal Fluid)

Simple Plex Human Myeloperoxidase/MPO Cartridge Summary

Simple Plex assay for the detection of human Myeloperoxidase (MPO) in cell culture supernatant (CCS), serum, platelet-poor plasma (EDTA/Heparin), urine, and cerebrospinal fluid (CSF).

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all Myeloperoxidase/MPO Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
Learn More

Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human MPO.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay.

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents.

Cell Culture Supernates, Cerebrospinal Fluid, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Serum, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 17 19
Mean (pg/mL) 178 8592 155 619
Standard Deviation 11.2 633 14.0 714
CV% 6.3 7.4 9.0 9.4

Recovery for Simple Plex Human Myeloperoxidase/MPO Cartridge

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Samples (n=2) 104 102-106

Linearity

Samples containing and/or spiked with high concentrations of human MPO were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Human Myeloperoxidase/MPO Ella Assay Linearity

Scientific Data Images for Simple Plex Human Myeloperoxidase/MPO Cartridge

Human Myeloperoxidase/MPO Ella Assay Standard Curve

Human Myeloperoxidase/MPO Ella Assay Standard Curve

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Myeloperoxidase/MPO

Myeloperoxidase (MPO) is a heme-containing enzyme belonging to the XPO subfamily of peroxidases. It is an abundant neutrophil and monocyte glycoprotein that catalyzes the hydrogen peroxide dependent formation of hypochlorus acid (HOCl) and other reactive species. Enzymatically active MPO is a disulfide-linked tetramer that contains two heme groups and two copies each of the heavy and light chains. MPO binds Albumin, MMR, Cytokeratin 1 on vascular endothelial cells, HMW Kininogen, and Integrin CD11b/CD18 on neutrophils. These interactions promote MPO clearance, a reduction of nitric oxide and bradykinin levels, reduced vasodilation, and continued neutrophil activation. Neutrophil MPO is stored in cytoplasmic azurophilic granules. Upon cellular activation and degranulation, MPO is delivered into phagosomes where it is required for the killing of phagocytosed bacteria. Activated neutrophils also release granule contents extracellularly. Elevated plasma MPO levels have been associated with a variety of clinical conditions including systemic inflammation, eclampsia, risk of cardiovascular events, vascular endothelial dysfunction, severity of multiple sclerosis, and prospective mortality and oxidative stress during hemodialysis.

Alternate Names

MPO

Entrez Gene IDs

4353 (Human); 17523 (Mouse); 303413 (Rat)

Gene Symbol

MPO

Additional Myeloperoxidase/MPO Products

Product Documents for Simple Plex Human Myeloperoxidase/MPO Cartridge

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Certificate of Analysis

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Product Specific Notices for Simple Plex Human Myeloperoxidase/MPO Cartridge

For research use only

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