Simple Plex Human Neprilysin/CD10 Cartridge

Name: Simple Plex Human Neprilysin/CD10 Cartridge
Brand: R&D Systems
SKU: SPCKB-PS-001519
Price: 450 USD
Availability: InStock

R&D Systems | Catalog # SPCKB-PS-001519

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Product Documents

Simple Plex Human Neprilysin/CD10 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.

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Compatible Instruments
Product Specifications
Precision
Recovery
Linearity
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL)

Sensitivity

0.82 pg/mL

Assay Range

11.8-18000 pg/mL (Serum, EDTA Plasma, Heparin Plasma)

Simple Plex Human Neprilysin/CD10 Cartridge Summary

Simple Plex assay is for the detection of human Neprilysin in serum and plasma (EDTA/Heparin) in Sample Diluent SD13.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

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Compatible Instruments

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.

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Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human Neprilysin.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one Neprilysin assay.

Inter-Assay Precision (Precision between assays) Replicates of each Neprilysin control were tested in multiple assays performed by at least three technicians using two lots of reagents for human Neprilysin.

EDTA Plasma, Heparin Plasma, Serum

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	56	59
Mean (pg/mL)	114	5616	123	6348
Standard Deviation	6.41	326	11.8	611
CV%	5.6	5.8	9.6	9.6

Recovery for Simple Plex Human Neprilysin/CD10 Cartridge

Recovery for the human Neprilysin assays was evaluated at three different spiked concentrations of Neprilysin protein.

Sample Type	Average % Recovery	Range %
EDTA Plasma (n=4)	100	96-103
Heparin Plasma (n=4)	97	91-103
Serum (n=4)	96	93-100

Linearity

Samples containing and/or spiked with high concentrations of human Neprilysin were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data Images for Simple Plex Human Neprilysin/CD10 Cartridge

Simple Plex Human Neprilysin Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human Neprilysin Assay. Assay range is 11.8-18,000 pg/mL.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Neprilysin/CD10

The neprilysin (NEP) family of zinc metallopeptidases includes neprilysin, endothelin-converting enzyme-2 (ECE-2), PEX, damage induced neuronal endopeptidase (DINE), Kell and several neprilysin-like proteins. Neprilysin is expressed at the cell surface of a variety of cell types. Enzymatically, neprilysin functions both as an endopeptidase with a thermolysin-like specificity and as a dipeptidylcarboxypeptidase. Neprilysin has been shown to be involved in the degradation of enkephalins in the mammalian brain and the inactivation of circulating atrial natriuretic peptide. Neprilysin has also been identified as the common acute lymphoblastic leukemia antigen (CALLA), and to be expressed on the surface of lymphocytes in some disease states. These and other observations have resulted in considerable clinical interest in neprilysin as a potential target for analgesics and antihypertensive drugs. Neprilysin is also a major degrading enzyme of amyloid-beta peptide (A-beta) in the brain, indicating that down-regulation of neprilysin activity, which could be caused by aging, can contribute to the development of Alzheimers disease by promoting A-beta accumulation.