Simple Plex Human NGFR/TNFRSF16 Cartridge

Name: Simple Plex Human NGFR/TNFRSF16 Cartridge
Brand: R&D Systems
SKU: SPCKB-PS-000297
Price: 450 USD
Availability: InStock

R&D Systems | Catalog # SPCKB-PS-000297

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Product Documents

Simple Plex Human NGFR/TNFRSF16 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.

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Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and up to 8 analytes per cartridge. Customize this cartridge.

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Compatible Instruments
Product Specifications
Precision
Recovery
Linearity
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL), Urine (25 µL)

Sensitivity

12.7 pg/mL

Assay Range

28.6-108900 pg/mL (Serum, EDTA Plasma, Heparin Plasma, Urine)

Simple Plex Human NGFR/TNFRSF16 Cartridge Summary

Simple Plex assay is for the detection of human Nerve Growth Factor Receptor (NGFR)/TNFRSF16 in serum, plasma (EDTA/Heparin), and urine in Sample Diluent SD13.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

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Compatible Instruments

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.

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Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human NGFR.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one NGFR assay.

Inter-Assay Precision (Precision between assays) Replicates of each NGFR control were tested in multiple assays performed by at least three technicians using two lots of reagents for human NGFR.

EDTA Plasma, Heparin Plasma, Serum, Urine

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	9	8
Mean (pg/mL)	613	32,027	597	35,057
Standard Deviation	28.5	2331	39.1	2601
CV%	4.7	7.3	6.5	7.4

Recovery for Simple Plex Human NGFR/TNFRSF16 Cartridge

Recovery for the human NGFR assays was evaluated at three different spiked concentrations of NGFR protein.

Sample Type	Average % Recovery	Range %
EDTA Plasma (n=4)	112	101-122
Heparin Plasma (n=4)	106	88-119
Serum (n=4)	108	98-115

Linearity

Samples containing and/or spiked with high concentrations of human NGF R were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data Images for Simple Plex Human NGFR/TNFRSF16 Cartridge

Simple Plex Human NGFR Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human NGFR Assay. Assay range is 28.6-108,900 pg/mL.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: NGFR/TNFRSF16

The low affinity nerve growth factor receptor (NGFR), is a type I transmembrane protein that belongs to the tumor necrosis factor receptor family1 and has been designated TNFRSF16. The NGF receptor is also known as p75 NTR (neurotrophin receptor) because of its ability to bind at low affinity not only to NGF, but also other neurotrophins including brain-derived neurotrophic factor (BDNF), neurotrophin-3 and neurotrophin-4/5. NGFR also binds several myelin derived proteins (Nogo, myelin associated glycoprotein, and myelin oligodendrocyte glycoprotein) as a co-receptor with Nogo receptor.

The NGF receptor is a 75 kDa protein that is expressed in neuronal axons, Schwanns cells and perineural cells of peripheral nerves. Neural crest stem cells have been isolated based on their surface expression of NGFR. In addition, neuroepithelial-derived NGFR-positive cells have also been demonstrated to be able to differentiate into neurons, smooth muscle and Schwann cells in culture. The expression of NGFR has also been used as a marker to identify mesenchymal precursors as well as hepatic stellate cells.