Best Seller

Simple Plex Human Proprotein Convertase 9/PCSK9 Cartridge

R&D Systems | Catalog # SPCKB-PS-000321

Simple Plex Human Proprotein Convertase 9/PCSK9 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.
R&D Systems

Customize This Cartridge

Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and up to 8 analytes per cartridge. Customize this cartridge.

Customize This Cartridge

Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL)

Sensitivity

4.32 pg/mL

Assay Range

14.9-56970 pg/mL (Serum, EDTA Plasma, Heparin Plasma)

Simple Plex Human Proprotein Convertase 9/PCSK9 Cartridge Summary

Simple Plex Human PCSK9 Assay is for the detection of Proprotein Convertase 9 (PCSK9) in serum and plasma (EDTA/Heparin) in Sample Diluent SD13.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all Proprotein Convertase 9/PCSK9 Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
Learn More

Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human PCSK9.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one PCSK9 assay.

Inter-Assay Precision (Precision between assays) Replicates of each PCSK9 control were tested in multiple assays performed by at least three technicians using two lots of reagents for human PCSK9.

EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 22 22
Mean (pg/mL) 296 17,116 292 17,472
Standard Deviation 19.6 842 18.3 1101
CV% 6.6 4.9 6.3 6.3

Recovery for Simple Plex Human Proprotein Convertase 9/PCSK9 Cartridge

Recovery was not necessary as human PCSK9 shows 5 points of natural linearity.

Linearity

Samples containing and/or spiked with high concentrations of human PCSK9 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Simple Plex Human PCSK9 Assay Linearity

Scientific Data Images for Simple Plex Human Proprotein Convertase 9/PCSK9 Cartridge

Simple Plex Human PCSK9 Assay Standard Curve

Simple Plex Human PCSK9 Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human PCSK9 Assay. Assay range is 14.9-56,970 pg/mL.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Proprotein Convertase 9/PCSK9

PCSK9 (Proprotein Convertase 9, is a serine endoprotease that is highly expressed in the liver, intestine, and kidney. PCSK9 promotes the degradation of low density lipoprotein receptor (LDLR) by inducing receptor degradation in the lysosome following its internalization. PCSK9 regulates the amounts of LDLR in the circulation and hence, modulates cholesterol levels. Nonsense or loss-of-function mutations in PCSK9 are associated with decreased plasma LDL cholesterol levels.

Alternate Names

FH3, FHCL3, HCHOLA3, LDLCQ1, NARC-1, NARC1, PC9, PCSK9

Entrez Gene IDs

255738 (Human); 100102 (Mouse); 298296 (Rat); 102142788 (Cynomolgus Monkey)

Gene Symbol

PCSK9

Additional Proprotein Convertase 9/PCSK9 Products

Product Documents for Simple Plex Human Proprotein Convertase 9/PCSK9 Cartridge

Loading product document information...

Loading product document information...

Certificate of Analysis

To download a Certificate of Analysis, please enter your Simple Plex catalog number and lot number in the search boxes below.

Product Specific Notices for Simple Plex Human Proprotein Convertase 9/PCSK9 Cartridge

For research use only

Related Research Areas

Obesity
Serine Proteases and Regulators

Citations for Simple Plex Human Proprotein Convertase 9/PCSK9 Cartridge

View all citations for the Simple Plex platform.

Please visit our Instrument Citation Database to search our collection of scientific articles that feature our instruments.

Customer Reviews for Simple Plex Human Proprotein Convertase 9/PCSK9 Cartridge

There are currently no reviews for this product. Be the first to review Simple Plex Human Proprotein Convertase 9/PCSK9 Cartridge and earn rewards!

Have you used Simple Plex Human Proprotein Convertase 9/PCSK9 Cartridge?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card