Simple Plex Human TNF RI/TNFRSF1A Cartridge

Name: Simple Plex Human TNF RI/TNFRSF1A Cartridge
Brand: R&D Systems
SKU: SPCKB-PS-000434
Price: 450 USD
Availability: InStock

R&D Systems | Catalog # SPCKB-PS-000434

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Product Documents

Simple Plex Human TNF RI/TNFRSF1A assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.

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Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and up to 8 analytes per cartridge. Customize this cartridge.

Customize This Cartridge

Compatible Instruments
Product Specifications
Precision
Recovery
Linearity
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (25 µL), Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL), Urine (25 µL), Cerebrospinal Fluid (25 µL)

Sensitivity

0.1 pg/mL

Assay Range

0.89-3390 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Cerebrospinal Fluid)

Simple Plex Human TNF RI/TNFRSF1A Cartridge Summary

Simple Plex assay is for the detection of human Tumor Necrosis Factor Receptor I (TNF RI) in cell culture supernatant (CCS), serum, plasma (EDTA/Heparin), urine, and cerebrospinal fluid (CSF) in Sample Diluent SD13.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all TNF RI/TNFRSF1A Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.

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Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human TNF RI.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Multiple replicates of each control were tested in one assay.

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple runs performed by at least three technicians using two lots of reagents.

Cell Culture Supernates, Cerebrospinal Fluid, EDTA Plasma, Heparin Plasma, Serum, Urine

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	24	22
Mean (pg/mL)	20.1	963	19.5	971
Standard Deviation	0.748	63.5	2.30	97.5
CV%	3.7	6.6	11.8	10.0

Recovery for Simple Plex Human TNF RI/TNFRSF1A Cartridge

Recovery was not necessary as TNF RI shows 5 points of natural linearity.

Linearity

Samples containing and/or spiked with high concentrations of human TNF RI were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data Images for Simple Plex Human TNF RI/TNFRSF1A Cartridge

Human TNF RI/TNFRSF1A Ella Assay Standard Curve

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: TNF RI/TNFRSF1A

TNF RI (TNF receptor 1; TNF R-p55/p60) is a transmembrane receptor for TNF-alpha and Lymphotoxin-alpha/TNF-beta. A soluble decoy form of the receptor is shed by ADAM-17/TACE in response to inflammatory stimulation. TNF RI signaling through NFkB is essential for the development of lymph node germinal centers and Peyerâ€™s patches and for combating intracellular pathogens such as Listeria.