Simple Plex Human TNF RI/TNFRSF1A Cartridge

Catalog #: SPCKB-PS-000434 Datasheet / COA / SDS
Simple Plex Human TNF RI/TNFRSF1A assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer
Catalog # Availability Size / Price Qty
SPCKB-PS-000434
SPCKA-PS-000434
SPCKC-PS-000434
Control Products Available
Human TNF RI/TNFRSF1A Simple Plex Assay
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Simple Plex Human TNF RI/TNFRSF1A Cartridge Summary

Sample Type & Volume Required Per Well*
Cell Culture Supernates (25 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL), Cerebrospinal Fluid (25 uL)
Sensitivity
0.89 pg/mL
Assay Range
0.9 - 3,390 pg/mL (Serum, EDTA Plasma, Heparin Plasma, Cerebrospinal Fluid)
Specificity
Natural and recombinant human TNF RI.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

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Product Summary

Simple Plex assay for the detection of human TNF RI in cell culture supernatan (CCS), serum, plasma (EDTA/Heparin), and cerebrospinal fluid (CSF)

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Cerebrospinal Fluid

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 16 16
Mean (pg/mL) 20.1 963 19.5 971
Standard Deviation 0.748 63.5 2.3 97.5
CV% 3.7 6.6 11.8 10

Recovery

Recovery was not necessary as TNF RI shows natural linearity data from a neat sample to a 1:16 dilution.

Linearity

Samples containing and/or spiked with high concentrations of human TNF RI were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
Human TNF RI Simple Plex Assay Linearity

Scientific Data

Human TNF RI Simple Plex Assay Standard Curve

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Preparation and Storage

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: TNF RI/TNFRSF1A

TNF RI (TNF receptor 1; TNF R-p55/p60) is a transmembrane receptor for TNF-alpha and Lymphotoxin-alpha/TNF-beta. A soluble decoy form of the receptor is shed by ADAM-17/TACE in response to inflammatory stimulation. TNF RI signaling through NFkB is essential for the development of lymph node germinal centers and Peyer’s patches and for combating intracellular pathogens such as Listeria.

Long Name:
Tumor Necrosis Factor Receptor I
Entrez Gene IDs:
7132 (Human); 21937 (Mouse); 25625 (Rat); 102135285 (Cynomolgus Monkey)
Alternate Names:
CD120a antigen; CD120a; FPF; p55; p55-R; p60; TNF RI; TNFARMGC19588; TNF-R; TNF-R1; TNFR1TBP1; TNFR55; TNF-R55; TNFR60; TNFRI; TNF-RI; TNF-R-I; TNFR-I; TNFRSF1A; tumor necrosis factor binding protein 1; Tumor necrosis factor receptor 1; tumor necrosis factor receptor 1A isoform beta; tumor necrosis factor receptor superfamily member 1A; tumor necrosis factor receptor superfamily, member 1A; tumor necrosis factor receptor type 1; Tumor necrosis factor receptor type I; tumor necrosis factor-alpha receptor

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