Simple Plex Human VEGF Cartridge

R&D Systems | Catalog # SPCKB-PS-000330

Simple Plex Human VEGF assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer. CSF samples are validated in SD13 only, it is not recommended to use SD06.
R&D Systems

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Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and up to 8 analytes per cartridge. Customize this cartridge.

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Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (25 µL), Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL), Cerebrospinal Fluid (25 µL)

Sensitivity

0.28 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
1.75 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Cerebrospinal Fluid)

Assay Range

2.99-4562 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
3.65-5570 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Cerebrospinal Fluid)

Simple Plex Human VEGF Cartridge Summary

Simple Plex assay for the detection of human Vascular Endothelial Growth Factor A (VEGF-A) in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin), and cerebrospinal fluid (CSF). CSF samples are validated in SD13 only, it is not recommended to use SD06.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all VEGF Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
Learn More

Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human (rh) VEGF111, rhVEGF121, rhVEGF145, rhVEGF165, and rhVEGF189. 

Cross-reactivity

Cross-reactivity observed with 1 or more available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay.

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents.

Cell Culture Supernates, Cerebrospinal Fluid, EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 8 10
Mean (pg/mL) 34.5 1519 33.3 1440
Standard Deviation 1.32 109 2.38 89.6
CV% 3.8 7.1 7.1 6.2

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 8 10
Mean (pg/mL) 26.1 1126 1.33 72.3
Standard Deviation 1.33 72.3 1.90 139
CV% 5.1 6.4 6.6 10.8

Recovery for Simple Plex Human VEGF Cartridge

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cerebrospinal Fluid (n=6) 114 110-119
EDTA Plasma (n=3) 91 82-100
Heparin Plasma (n=3) 86 75-94
Serum (n=3) 95 82-106

Linearity

Samples containing and/or spiked with high concentrations of human VEGF-A were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Images for Simple Plex Human VEGF Cartridge

Human VEGF Ella Assay Standard Curve (Sample Diluent 13)

Human VEGF Ella Assay Standard Curve (Sample Diluent 06)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: VEGF

VEGF (Vascular endothelial growth factor) is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. It is required for regulating the proliferation, migration, and survival of embryonic endothelial cells and during wound healing and the female reproductive cycle in adults. Pathologically, it is involved in tumor development and tumor vascular leakage. VEGF binds to VEGF R1/Flt-1, VEGF R2/Flk-1/KDR, and Neuropilin-1. Multiple forms of VEGF are generated by alternative splicing and proteolysis.

Long Name

Vascular Endothelial Growth Factor

Alternate Names

MVCD1, VAS, Vasculotropin, VEGF-A, VEGFA, VPF

Entrez Gene IDs

7422 (Human); 22339 (Mouse); 83785 (Rat); 281572 (Bovine); 403802 (Canine); 493845 (Feline); 30682 (Zebrafish)

Gene Symbol

VEGFA

Additional VEGF Products

Product Documents for Simple Plex Human VEGF Cartridge

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Certificate of Analysis

To download a Certificate of Analysis, please enter your Simple Plex catalog number and lot number in the search boxes below.

Product Specific Notices for Simple Plex Human VEGF Cartridge

For research use only

Citations for Simple Plex Human VEGF Cartridge

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  • Simple Plex Human VEGF Cartridge
    Name: Sara Pelucchi
    Sample Tested: Serum and Plasma
    Verified Customer | Posted 05/09/2023
    Simple Plex Human VEGF Cartridge SPCKB-PS-000330

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