Simple Plex Mouse CCL3/MIP-alpha Cartridge

Name: Simple Plex Mouse CCL3/MIP-alpha Cartridge
Brand: R&D Systems
SKU: SPCKB-MP-007987
Price: 450 USD
Availability: InStock

R&D Systems | Catalog # SPCKB-MP-007987

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Product Documents

Simple Plex Mouse CCL3/MIP-1 alpha assay kit for use on Ella instrument. Contains cartridge, sample diluent SD09, and wash buffer.

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Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and up to 8 analytes per cartridge. Customize this cartridge.

Customize This Cartridge

Compatible Instruments
Product Specifications
Precision
Recovery
Linearity
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 µL), Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL), Bronchoalveolar Lavage Fluid (25 µL)

Sensitivity

0.02 pg/mL

Assay Range

0.26-400 pg/mL (Bronchoalveolar Lavage Fluid, Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

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Compatible Instruments

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.

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Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Mouse

Specificity

Natural and recombinant mouse MIP-1 alpha.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay.

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents.

Bronchoalveolar Lavage Fluid, Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	117	117
Mean (pg/mL)	2.57	139	2.98	149
Standard Deviation	0.131	3.82	0.283	11.0
CV%	5.1	2.7	9.5	7.4

Recovery for Simple Plex Mouse CCL3/MIP-alpha Cartridge

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
EDTA Plasma (n=4)	96	81-95
Heparin Plasma (n=4)	84	78-90
Serum (n=4)	99	95-105

Linearity

Scientific Data Images for Simple Plex Mouse CCL3/MIP-alpha Cartridge

Mouse CCL3/MIP-1 alpha Ella Assay Standard Curve

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CCL3/MIP-1 alpha

CCL3/MIP-1 alpha, is a beta chemokine that can be induced in a variety of hematopoietic cells, fibroblasts, smooth muscle cells, and epithelial cells. It forms complexes with sulfated proteoglycans and also associates into noncovalently-linked dimers and high molecular weight polymers. These complexes of CCL3 are protected from proteolytic digestion by insulin degrading enzyme (IDE) which can cleave the monomeric chemokine. CCL3 signals through CCR1, CCR3, and CCR5, and it is internalized by the decoy chemokine receptor D6. CCL3 promotes the chemoattraction, adhesion to activated vascular endothelium, and cellular activation of many hematopoietic cell types. It can promote tumor metastasis, hyperalgesia, and inflammation and suppress HIV replication and hematopoietic stem cell proliferation.