Simple Plex Mouse CXCL12/SDF-1 alpha Cartridge
R&D Systems | Catalog # SPCKB-MP-001192
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Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and up to 8 analytes per cartridge. Customize this cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Mouse CXCL12/SDF-1 alpha Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Species
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one CXCL12/SDF-1 alpha assay.
Inter-Assay Precision (Precision between assays) Replicates of each CXCL12/SDF-1 alpha control were tested in multiple assays performed by at least three technicians using two lots of reagents for mouse CXCL12/SDF-1 alpha.
Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 21 | 22 |
| Mean (pg/mL) | 96.1 | 4737 | 91.9 | 4984 |
| Standard Deviation | 9.57 | 389 | 7.36 | 350 |
| CV% | 10.0 | 8.2 | 8.0 | 7.0 |
Recovery for Simple Plex Mouse CXCL12/SDF-1 alpha Cartridge
Recovery was not necessary as mouse CXCL12/SDF-1 alpha shows 5 points of natural linearity.
Linearity
Samples containing and/or spiked with high concentrations mouse CXCL12/SDF-1 alpha were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
Scientific Data Images for Simple Plex Mouse CXCL12/SDF-1 alpha Cartridge
Simple Plex Mouse CXCL12/SDF-1 alpha Assay Standard Curve
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Mouse CXCL12/SDF-1 alpha Assay. Assay range is 26.0-15,860 pg/mL.Preparation and Storage
Shipping
Stability & Storage
Background: CXCL12/SDF-1 alpha
CXCL12 is a heparin-binding chemokine that regulates lymphopoiesis, enhances the survival of myeloid progenitor cells, regulates the patterning and cell number of neural progenitors, and promotes angiogenesis. CXCL12/SDF-1 alpha is one of several SDF-1 isoforms, all of which can undergo proteolytic processing of the first two N-terminal amino acids by DPPIV/CD26. SDF-1 isoforms signal through CXCR4 and CXCR7 and can also bind Syndecan-4. Their binding to CXCR4 may block the cellular entry of HIV.
Alternate Names
Gene Symbol
Additional CXCL12/SDF-1 alpha Products
Product Documents for Simple Plex Mouse CXCL12/SDF-1 alpha Cartridge
Certificate of Analysis
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Product Specific Notices for Simple Plex Mouse CXCL12/SDF-1 alpha Cartridge
For research use only
Related Research Areas
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Associated Pathways
Jak/STAT Signaling Pathway
