Simple Plex Mouse IL-1 ra/IL-1F3 Cartridge
R&D Systems | Catalog # SPCKB-MP-004019
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Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and up to 8 analytes per cartridge. Customize this cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Mouse IL-1 ra/IL-1F3 Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Species
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one IL-1 ra/IL-1F3 assay.
Inter-Assay Precision (Precision between assays) Replicates of each IL-1 ra/IL-1F3 control were tested in multiple assays performed by at least three technicians using two lots of reagents for mouse IL-1 ra/IL-1F3.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 91 | 88 |
| Mean (pg/mL) | 110 | 4905 | 88.6 | 3848 |
| Standard Deviation | 3.03 | 153 | 10.5 | 412 |
| CV% | 2.8 | 3.1 | 11.8 | 10.7 |
Recovery for Simple Plex Mouse IL-1 ra/IL-1F3 Cartridge
Recovery was not necessary as mouse IL-1 ra/IL-1F3 shows 5 points of natural linearity.
Linearity
Samples containing and/or spiked with high concentrations of mouse IL-1 ra/IL-1F3 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Simple Plex Mouse IL-1 ra/IL-1F3 Cartridge
Simple Plex Mouse IL-1 ra/IL-1F3 Assay Standard Curve
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Mouse IL-1 ra/IL-1F3 Assay. Assay range is 6.43-9806 pg/mL.Preparation and Storage
Shipping
Stability & Storage
Background: IL-1ra/IL-1F3
IL-1ra (IL-1 receptor antagonist) is a widely expressed acute phase protein that serves to dampen inflammation. IL-1ra binds to both IL-1 RI and IL-1 RII. It competitively inhibits IL-1 (alpha or beta) binding to IL-1 RI and does not trigger recruitment of the accessory molecule IL-1 R AcP or activation of IL-1 RI signaling. IL-1ra blocks the proinflammatory actions of IL-1 including Prostaglandin E2 and IL-6 release from endothelial cells, fever generation, thrombocytosis, the production of hepatic acute phase proteins, and neutrophil expansion and infiltration.
Long Name
Alternate Names
Gene Symbol
Additional IL-1ra/IL-1F3 Products
Product Documents for Simple Plex Mouse IL-1 ra/IL-1F3 Cartridge
Certificate of Analysis
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Product Specific Notices for Simple Plex Mouse IL-1 ra/IL-1F3 Cartridge
For research use only
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Associated Pathways
