Simple Plex Mouse/Rat VEGF-A Cartridge

Name: Simple Plex Mouse/Rat VEGF-A Cartridge
Brand: R&D Systems
SKU: SPCKB-MP-006691
Price: 450 USD
Availability: InStock

R&D Systems | Catalog # SPCKB-MP-006691

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Product Documents

Simple Plex Mouse/Rat VEGF assay kit for use on Ella instrument. Contains cartridge, sample diluent SD09, and wash buffer.

Customize This Cartridge

Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and up to 8 analytes per cartridge. Customize this cartridge.

Customize This Cartridge

Compatible Instruments
Product Specifications
Precision
Recovery
Linearity
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas
Related Blogs

Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL)

Sensitivity

0.5 pg/mL

Assay Range

0.91-3478 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Simple Plex Mouse/Rat VEGF-A Cartridge Summary

Simple Plex assay is for the detection of mouse and rat Vascular Endothelial Growth Factor (VEGF-A) in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin), in Sample Diluent SD09.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

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Compatible Instruments

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.

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Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Mouse, Rat

Specificity

Natural rat and mouse VEGF-A and recombinant mouse (rm) VEGF120, rmVEGF188, and rmVEGF164.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

Interference observed with 1 or more available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one VEGF-A assay.

Inter-Assay Precision (Precision between assays) Replicates of each VEGF-A control were tested in multiple assays performed by at least three technicians using two lots of reagents for VEGF-A.

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	54	55
Mean (pg/mL)	24.6	1016	21.8	985
Standard Deviation	1.09	60.3	1.97	78.9
CV%	4.4	5.9	9.0	8.0

Recovery for Simple Plex Mouse/Rat VEGF-A Cartridge

Recovery for the mouse/rat VEGF-A assays was evaluated at three different spiked concentrations of VEGF-A protein.

Sample Type	Average % Recovery	Range %
Mouse EDTA Plasma (n=4)	96	94-100
Mouse Heparin Plasma (n=4)	91	89-93
Mouse Serum (n=4)	82	79-83
Rat EDTA Plasma (n=4)	92	88-95
Rat Heparin Plasma (n=4)	87	81-91
Rat Serum (n=4)	91	87-97

Linearity

Samples containing and/or spiked with high concentrations of mouse/rat VEGF-A were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Images for Simple Plex Mouse/Rat VEGF-A Cartridge

Simple Plex Mouse/Rat VEGF-A Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Mouse/Rat VEGF-A Assay. Assay range is 0.91-3478 pg/mL.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: VEGF

VEGF (Vascular endothelial growth factor) is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. It is required for regulating the proliferation, migration, and survival of embryonic endothelial cells and during wound healing and the female reproductive cycle in adults. Pathologically, it is involved in tumor development and tumor vascular leakage. VEGF binds to VEGF R1/Flt-1, VEGF R2/Flk-1/KDR, and Neuropilin-1. Multiple forms of VEGF are generated by alternative splicing and proteolysis.