Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all SLAIN1 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 518 and 568 of human SLAIN Motif Family, Member 1 using the numbering given in entry NP_001035243.1 (GeneID 122060).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SLAIN1 Antibody - BSA Free

Western Blot: SLAIN1 Antibody [NBP2-04062]

Western Blot: SLAIN1 Antibody [NBP2-04062]

Western Blot: SLAIN1 Antibody [NBP2-04062] - Detection of human SLAIN1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HEK293T(15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), Jurkat (J; 50 ug) and HeLa (H; 50 ug) cells. Antibodies: Affinity purified rabbit anti-SLAIN1 antibody NBP2-04062 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. SLAIN1 was also immunoprecipitated by another rabbit anti-SLAIN1 antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).

Applications for SLAIN1 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2-10 mcg/mg

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: SLAIN1

SLAIN1 (SLAIN motif-containing protein 1) is a 61 kDa (predicted) member of the SLAIN motif containing family of proteins. It is expressed in embryonic stem cells plus cells of the neural tube, and also appears in multiple adult tissues. Its function is unclear. Mouse SLAIN1 is 579 amino acids (aa) in length. It contains a coiled-coil region (aa 21-56), a Pro-rich segment (aa 64-160), and one C-terminal SLAIN (SerLeuAlaIleAsn) motif. There are two potential splice variants. One shows an alternate start site at Met262, while another shows a 50 aa insertion after Gln231. Over aa 262-483, mouse SLAIN1 shares 96% and 92% aa identity with rat and human SLAIN1, respectively.

Long Name

Slain motif-containing Protein 1

Alternate Names

SLAIN motif family, member 1

Gene Symbol

SLAIN1

UniProt

NP_001035243.1 (Human)

Additional SLAIN1 Products

Product Documents for SLAIN1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for SLAIN1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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