SLC25A28 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-59562

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

Synthetic peptides corresponding to SLC25A28(solute carrier family 25, member 28) The peptide sequence was selected from the C terminal of SLC25A28. Peptide sequence NTQESLALNSHITGHITGMASAFRTVYQVGGVTAYFRGVQARVIYQIPST. The peptide sequence for this immunogen was taken from within the described region.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Scientific Data Images for SLC25A28 Antibody - BSA Free

Western Blot: SLC25A28 Antibody [NBP1-59562]

Western Blot: SLC25A28 Antibody [NBP1-59562]

Western Blot: SLC25A28 Antibody [NBP1-59562] - Titration: 1 ug/ml Positive Control: MCF-7 whole cell lysates.
Immunohistochemistry-Paraffin: SLC25A28 Antibody [NBP1-59562]

Immunohistochemistry-Paraffin: SLC25A28 Antibody [NBP1-59562]

Immunohistochemistry-Paraffin: SLC25A28 Antibody [NBP1-59562] - Human kidney Tissue, antibody concentration 5 ug/ml.
SLC25A28 Antibody - BSA Free

Western Blot: SLC25A28 Antibody - BSA Free [NBP1-59562] -

Iron content and iron-related transport proteins in cardiac tissue and mitochondria during CIH. (A) Perls’ staining of heart tissue (scale bar = 25 μm). (B) The mean density of Fe content as shown in panel A. (C,D) The expression and statistics of the iron-related transport proteins, TfR1, DMT1(−ire), DMT1(+ire) and FPN1. (E,F) The expression and statistics of iron transporter-associated proteins in mitochondria, Mfrn2, MtFt and ABCB8. The results are presented as the mean +/- SEM, n = 3. ∗p < 0.05, **p < 0.01 vs. Con group. #p < 0.05 vs. BHD-M group. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35286247), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for SLC25A28 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1.0 ug/ml

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 2% Sucrose

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SLC25A28

SLC25A28 is a mitochondrial iron transporter that mediates iron uptake. It is probably required for heme synthesis of hemoproteins and Fe-S cluster assembly in non-erythroid cells. The iron delivered into the mitochondria, presumably as Fe(2+), is then probably delivered to ferrochelatase to catalyze Fe(2+) incorporation into protoprophyrin IX to make heme.

Long Name

solute carrier family 25 member 28

Alternate Names

MFRN2, MRS3/4, MRS4L, NPD016

Gene Symbol

SLC25A28

UniProt

Additional SLC25A28 Products

Product Documents for SLC25A28 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for SLC25A28 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for SLC25A28 Antibody - BSA Free

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Protocols

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