SLC26A4 Antibody - BSA Free

A: You are right that human SLC26A4 has two isoforms: isoform 1: 780 a.a. with mass (Da):85,723 and isoform 2: 349 a.a. mass (Da):39,267. But, according to "http://www.uniprot.org/uniprot/O43511#sequences", this protein contains 12 transmembrane domains and should be treated very carefully when preparing lysates. There are two suggestions I have come by for these kind of membrane proteins: (1), use 1% SDS (instead of 0.1%) in the RIPA buffer and/or (2) don't boil but use room temperature for 30 min after adding SDS sample buffer, before loading to the SDS-PAGE. The multiple bands around both isoforms 1 and 2 are likely to be derived from the complexes that were still associated with lipid moieties of the membranes. Furthermore, try to test with cell lines, rather than directly on the tissue samples, as cell lines will usually have less complexity on the isoform expression patterns.