Q: Could you kindly provide published references for Western blot in detection of Dopamine transporter in human brain tissue with your antibody/antibodies?

A: Catalog numbers NBP2-22164, NB300-254 and NB100-65459 are well characterized DAT1 antibodies that have been referenced. If you refer to our website we have links to the PMID listing for each reference on the individual datasheets.

Human SLC6A3/DAT1 ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP2-67271

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Product Specifications
Precision
Recovery
Linearity
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

0.10 ng/mL

Assay Range

0.16 - 10 ng/mL

View all SLC6A3/DAT1 ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Human

Specificity

This kit recognizes Human DAT in samples. No significant cross-reactivity or interference between Human DAT and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% 5.87%

Inter-Assay Precision (Precision between assays) CV% 5.42%

Recovery for Human SLC6A3/DAT1 ELISA Kit (Colorimetric)

Recovery

86-110%

Linearity

Scientific Data Images for Human SLC6A3/DAT1 ELISA Kit (Colorimetric)

ELISA: Human SLC6A3/DAT1 ELISA Kit (Colorimetric) [NBP2-67271] -

ELISA: Human SLC6A3/DAT1 ELISA Kit (Colorimetric) [NBP2-67271] - Standard Curve Reference

Kit Contents for Human SLC6A3/DAT1 ELISA Kit (Colorimetric)

Biotinylated Detection Ab Diluent
Concentrated Biotinylated Detection Ab (100x)
Concentrated HRP Conjugate (100x)
Concentrated Wash Buffer (25x)
HRP Conjugate Diluent
Micro ELISA Plate (Dismountable)
Plate Sealer
Product Manual
Reference Standard
Sample Diluent
Stop Solution
Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: SLC6A3/DAT1

The Dopamine Transporter (DAT) is responsible for the reaccumulation of dopamine after it has been released. DAT antibodies and antibodies for other markers of catecholamine biosynthesis are widely used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson's disease and drug abuse (Kish et al., 2001; Zhu et al., 2000; Zhu et al., 1999). Levels of DAT protein expression are altered by chronic drug administration (Wilson et al., 1996).

Long Name

Dopamine Transporter

Alternate Names

DAT1, SLC6A3, VNTR

Gene Symbol

SLC6A3

Additional SLC6A3/DAT1 Products

Product Documents for Human SLC6A3/DAT1 ELISA Kit (Colorimetric)

Download Product Datasheets

Download Manual - Human SLC6A3/DAT1 ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Human SLC6A3/DAT1 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Related Research Areas

Neuronal Markers

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