Q: Could you kindly provide published references for Western blot in detection of Dopamine transporter in human brain tissue with your antibody/antibodies?

A: Catalog numbers NBP2-22164, NB300-254 and NB100-65459 are well characterized DAT1 antibodies that have been referenced. If you refer to our website we have links to the PMID listing for each reference on the individual datasheets.

SLC6A3/DAT1 293T Cell Transient Overexpression Lysate

Novus Biologicals | Catalog # H00006531-T02

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Product Summary
Product Specifications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species

Human

Applications

Western Blot

Product Summary for SLC6A3/DAT1 293T Cell Transient Overexpression Lysate

Quality control test: Transient overexpression cell lysate was tested with Anti-SLC6A3 antibody by Western Blots. Plasmid: pCMV-SLC6A3 full-length

Product Specifications

Specificity

SLC6A3/DAT1 293T Cell Transient Overexpression Lysate(Denatured)

Type

293T Cell Transient Overexpression

Protein State

Denatured

Formulation, Preparation, and Storage

Formulation

1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)

Concentration

mg/ml

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -80C. Avoid freeze-thaw cycles.

Background: SLC6A3/DAT1

The Dopamine Transporter (DAT) is responsible for the reaccumulation of dopamine after it has been released. DAT antibodies and antibodies for other markers of catecholamine biosynthesis are widely used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson's disease and drug abuse (Kish et al., 2001; Zhu et al., 2000; Zhu et al., 1999). Levels of DAT protein expression are altered by chronic drug administration (Wilson et al., 1996).

Long Name

Dopamine Transporter

Alternate Names

DAT1, SLC6A3, VNTR

Gene Symbol

SLC6A3

Additional SLC6A3/DAT1 Products

Product Documents for SLC6A3/DAT1 293T Cell Transient Overexpression Lysate

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Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for SLC6A3/DAT1 293T Cell Transient Overexpression Lysate

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Related Research Areas

Neuronal Markers

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