SLC7A5/LAT1 Antibody (EP3-1) - Chimeric - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-20116

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Functional

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # EP3-1

Format

Azide and BSA Free
View all SLC7A5/LAT1 Primary Antibodies »

Product Specifications

Immunogen

Immunised mice and constructed IgG1 Fab phage display libraries from the spleen cells. SLC7A5/LAT1-reactive recombinant Fab fragments were retrieved by epitope peptide panning.

Epitope

Shown to bind to epitopes of sequences; LLHGDFHAFSAG-GGGC-NH2, LLHGDFH-GGGC-HN2, and FHAFSAG-GGGC-NH2.

Specificity

Shows no reactivity to live HeLa cells by ICC/IF.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Scientific Data Images for SLC7A5/LAT1 Antibody (EP3-1) - Chimeric - Azide and BSA Free

SLC7A5/LAT1 Antibody (EP3-1) - Chimeric - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: SLC7A5/LAT1 Antibody (EP3-1) - Chimeric - Azide and BSA Free [NBP3-20116] -

Immunocytochemistry/Immunofluorescence: SLC7A5/LAT1 Antibody (EP3-1) - Chimeric - Azide and BSA Free [NBP3-20116] - Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells stained with the chimeric rabbit IgG version of EP3-1 (NBP3-20116) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-20116, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.
SLC7A5/LAT1 Antibody (EP3-1) - Chimeric - Azide and BSA Free

Flow Cytometry: SLC7A5/LAT1 Antibody (EP3-1) - Chimeric - Azide and BSA Free [NBP3-20116] -

Flow Cytometry: SLC7A5/LAT1 Antibody (EP3-1) - Chimeric - Azide and BSA Free [NBP3-20116] - Paraformaldehyde fixed MCF7 cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of EP3-1 (NBP3-20116, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Applications for SLC7A5/LAT1 Antibody (EP3-1) - Chimeric - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Functional

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Background: SLC7A5/LAT1

Mammalian cells have several amino acid transport systems. These systems have been defined by both activity and specific genes. L-type amino acid transporter 1 (LAT1) is a 12 membrane-spanning protein. LAT1 is a Na + -independent neutral amino acid transporter agency and essential for the transporter of large neutral amino acid such as Leucine, Isoleucine, and Valine through the plasma membrane. LAT1 has been proposed to be one of the major nutrient transport systems at the blood-brain barrier. Drugs such as L Dopa are transported by LAT1. LAT1 is thought to be up-regulated to support the high protein synthesis for cell growth in some tumor cell lines.

Long Name

Solute Carrier Family 7 Member 5

Alternate Names

4F2LC, CD98lc, E16, LAT1, MPE16, TA1

Gene Symbol

SLC7A5

Additional SLC7A5/LAT1 Products

Product Documents for SLC7A5/LAT1 Antibody (EP3-1) - Chimeric - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for SLC7A5/LAT1 Antibody (EP3-1) - Chimeric - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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