SLC7A5/LAT1 Antibody

Novus Biologicals | Catalog # NBP1-51935

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Canine (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all SLC7A5/LAT1 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-RHRKPELERPIKVN corresponding to internal region according to NP_003477.4.

Epitope

C-RHRKPELERPIKVN

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for SLC7A5/LAT1 Antibody

Western Blot: SLC7A5/LAT1 Antibody [NBP1-51935]

Western Blot: SLC7A5/LAT1 Antibody [NBP1-51935]

Western Blot: SLC7A5 Antibody [NBP1-51935] (0.3ug/ml) staining of Human Spleen lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for SLC7A5/LAT1 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:64000

Western Blot

1 - 3 ug/ml
Application Notes
WB: Approx. 60 kDa band observed in human spleen lysates (calculated MW of 55.0 kDa band according to NP_003477.4). Some minor background is detected and is blocked by the immunizing peptide. We call for caution when used for other assays than WB.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SLC7A5/LAT1

Mammalian cells have several amino acid transport systems. These systems have been defined by both activity and specific genes. L-type amino acid transporter 1 (LAT1) is a 12 membrane-spanning protein. LAT1 is a Na + -independent neutral amino acid transporter agency and essential for the transporter of large neutral amino acid such as Leucine, Isoleucine, and Valine through the plasma membrane. LAT1 has been proposed to be one of the major nutrient transport systems at the blood-brain barrier. Drugs such as L Dopa are transported by LAT1. LAT1 is thought to be up-regulated to support the high protein synthesis for cell growth in some tumor cell lines.

Long Name

Solute Carrier Family 7 Member 5

Alternate Names

4F2LC, CD98lc, E16, LAT1, MPE16, TA1

Entrez Gene IDs

8140 (Human)

Gene Symbol

SLC7A5

UniProt

NP_003477.4 (Human)

Additional SLC7A5/LAT1 Products

Product Documents for SLC7A5/LAT1 Antibody

Certificate of Analysis

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Product Specific Notices for SLC7A5/LAT1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for SLC7A5/LAT1 Antibody

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