SLPI Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-76803
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Immunogen
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Theoretical MW
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for SLPI Antibody - BSA Free
Western Blot: SLPI AntibodyBSA Free [NBP1-76803]
Western Blot: SLPI Antibody [NBP1-76803] - Daudi cell lysate with SLPI antibody at (A) 1 and (B) 2 ug/mL.Immunohistochemistry-Paraffin: SLPI Antibody - BSA Free [NBP1-76803]
Immunohistochemistry-Paraffin: SLPI Antibody [NBP1-76803] - Mouse lung tissue with SLPI antibody at 10 ug/ml.Immunocytochemistry/ Immunofluorescence: SLPI Antibody - BSA Free [NBP1-76803] -
SLPI is expressed in the bladder epithelium. (A and B) Immunofluorescence staining of SLPI protein expression (green) and DNA using Hoechst stain (blue) in mock-infected (A) and infected (B) bladders from Slpi+/+ mice. Left panels (5× magnification, 200 um scale bars) are merged images of SLPI and DNA shown as smaller panels on the right. (C–E) Mean fluorescence intensity (MFI) of mock and infected bladder epithelium for DNA (C) and SLPI (D) with MFI ratio of SLPI/DNA (E) (Student’s t test). (F) Comparison of supernatant SLPI levels in control (PBS) or infected (UTI89) 5637 bladder epithelial cells represented as percent of starting SLPI in each well. (G) Fold change in Slpi transcription as measured by qRT-PCR in 5637 cells at 2 hpi. For panels F and G, shapes denote separate biological replicate experiments, each point represents the average of 4 or more individual wells (paired Student’s t test). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/38270443), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Immunocytochemistry/ Immunofluorescence: SLPI Antibody - BSA Free [NBP1-76803] -
SLPI is expressed in the bladder epithelium. (A and B) Immunofluorescence staining of SLPI protein expression (green) and DNA using Hoechst stain (blue) in mock-infected (A) and infected (B) bladders from Slpi+/+ mice. Left panels (5× magnification, 200 um scale bars) are merged images of SLPI and DNA shown as smaller panels on the right. (C–E) Mean fluorescence intensity (MFI) of mock and infected bladder epithelium for DNA (C) and SLPI (D) with MFI ratio of SLPI/DNA (E) (Student’s t test). (F) Comparison of supernatant SLPI levels in control (PBS) or infected (UTI89) 5637 bladder epithelial cells represented as percent of starting SLPI in each well. (G) Fold change in Slpi transcription as measured by qRT-PCR in 5637 cells at 2 hpi. For panels F and G, shapes denote separate biological replicate experiments, each point represents the average of 4 or more individual wells (paired Student’s t test). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/38270443), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for SLPI Antibody - BSA Free
ELISA
Immunohistochemistry
Immunohistochemistry-Paraffin
Western Blot
Formulation, Preparation, and Storage
Purification
Formulation
Format
Preservative
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Stability & Storage
Background: SLPI
Long Name
Alternate Names
Gene Symbol
UniProt
Additional SLPI Products
Product Documents for SLPI Antibody - BSA Free
Certificate of Analysis
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Product Specific Notices for SLPI Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for SLPI Antibody - BSA Free
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Q: What is the molecular weight of SLPI?
A: Here is information about this protein, UniProt P03973. Its theoretical MW is approximately 14 kDa. In our western blot testing with this antibody, we picked up the band a little bit higher than this, which is could be due to post translational modification.