Smad2 [p Thr220] Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-04982
![Western Blot: Smad2 [p Thr220] Antibody [NBP1-04982]](https://resources.rndsystems.com/images/products/Smad2-[p-Thr220]-Antibody-Western-Blot-NBP1-04982-img0003.jpg "Western Blot: Smad2 [p Thr220] Antibody [NBP1-04982]")
Loading...
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Mouse
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Cited:
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Loading...
Product Specifications
Immunogen
Peptide sequence around phosphorylation site of threonine 220 (P-E-T(p)-P-P) derived from Human Smad2 (NP_001003652.1).
Modification
p Thr220
Specificity
Detects endogenous levels of Smad2 only when phosphorylated at threonine 220.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
52 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Smad2 [p Thr220] Antibody - BSA Free
![Smad2 [p Thr220] Antibody](https://resources.rndsystems.com/images/products/nbp1-04982_rabbit-polyclonal-smad2-p-thr220-antibody-234202415232511.jpg "Immunohistochemistry-Paraffin: Smad2 [p Thr220] Antibody [NBP1-04982] -")
Immunohistochemistry-Paraffin: Smad2 [p Thr220] Antibody [NBP1-04982] -
Immunohistochemistry-Paraffin: Smad2 [p Thr220] Antibody [NBP1-04982] - Immunohistochemical analysis of Smad2 staining in human brain formalin fixed paraffin embedded tissue section.![Smad2 [p Thr220] Antibody](https://resources.rndsystems.com/images/products/nbp1-04982_rabbit-polyclonal-smad2-p-thr220-antibody-234202414462213.jpg "Immunocytochemistry/ Immunofluorescence: Smad2 [p Thr220] Antibody [NBP1-04982] -")
Immunocytochemistry/ Immunofluorescence: Smad2 [p Thr220] Antibody [NBP1-04982] -
Immunocytochemistry/ Immunofluorescence: Smad2 [p Thr220] Antibody [NBP1-04982] - Immunofluorescent analysis of Smad2 staining in HeLa cells.![Smad2 [p Thr220] Antibody](https://resources.rndsystems.com/images/products/nbp1-04982_rabbit-polyclonal-smad2-p-thr220-antibody-23420241452107.jpg "Western Blot: Smad2 [p Thr220] Antibody [NBP1-04982] -")
Western Blot: Smad2 [p Thr220] Antibody [NBP1-04982] -
Western Blot: Smad2 [p Thr220] Antibody [NBP1-04982] - Western blot analysis of Smad2 [p Thr220] expression in rat heart (A) whole cell lysates.Applications for Smad2 [p Thr220] Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:500
Immunohistochemistry
1:50-1:200
Immunohistochemistry-Paraffin
1:10-1:500
Western Blot
1:500-1:1000
Application Notes
IHC-P reactivity reported in scientific literature (PMID: 23370707).
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol
Format
BSA Free
Preservative
0.01% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Smad2
Long Name
Mothers Against DPP Homolog 2
Alternate Names
JV18-1, MADH2, MADR2, Smox, pSMAD
Gene Symbol
SMAD2
UniProt
Additional Smad2 Products
Product Documents for Smad2 [p Thr220] Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Smad2 [p Thr220] Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for Smad2 [p Thr220] Antibody - BSA Free
Powered by Bioz
Customer Reviews for Smad2 [p Thr220] Antibody - BSA Free
There are currently no reviews for this product. Be the first to review Smad2 [p Thr220] Antibody - BSA Free and earn rewards!
Have you used Smad2 [p Thr220] Antibody - BSA Free?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
Loading...
Associated Pathways
Th17 Differentiation Pathway
