Q: I was wondering if you can recommend any primary polyclonal Smad3 antibody (reactivity: human) for gel supershifts?

A: We do provide a wide range of Smad3 antibodies, but unfortunately none of them have been tested for gel shift assay, so we can't guarantee they will work for an application never tested before.

Smad3 Antibody (PCRP-SMAD3-1A2)

Novus Biologicals | Catalog # NBP3-07255

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # PCRP-SMAD3-1A2

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Product Specifications

Immunogen

Recombinant full-length human Smad3 protein (Uniprot: P84022)

Localization

Cytoplasm, Nucleus

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Theoretical MW

54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08287)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for Smad3 Antibody (PCRP-SMAD3-1A2)

Immunocytochemistry/ Immunofluorescence: Smad3 Antibody (PCRP-SMAD3-1A2) [NBP3-07255]

Immunocytochemistry/Immunofluorescence: Smad3 Antibody (PCRP-SMAD3-1A2) [NBP3-07255] - Immunofluorescence Analysis of PFA-fixed HeLa cells using Smad3 Mouse Monoclonal Antibody (PCRP-SMAD3-1A2) followed by goat anti- Mouse IgG-CF488 (green). CF640A phalloidin (red).

Flow Cytometry: Smad3 Antibody (PCRP-SMAD3-1A2) [NBP3-07255]

Flow Cytometry: Smad3 Antibody (PCRP-SMAD3-1A2) [NBP3-07255] - Flow Cytometric Analysis of PFA-fixed HeLa cells. Smad3 Mouse Monoclonal Antibody (PCRP-Smad3-1A2) followed by goat anti- Mouse IgG-CF488 (blue); unstained cells (red).

Protein Array: Smad3 Antibody (PCRP-SMAD3-1A2) [NBP3-07255]

Protein Array: Smad3 Antibody (PCRP-SMAD3-1A2) [NBP3-07255] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Smad3 Mouse Monoclonal Antibody (PCRP-Smad3-1A2).

Applications for Smad3 Antibody (PCRP-SMAD3-1A2)

Application

Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Application Notes

Optimal dilution for a specific application should be determined.

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Advanced Features

Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
Spillover Popups - Visualize the spectra of individual fluorochromes
Antigen Density Selector - Match fluorochrome brightness with antigen density

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Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Smad3

The protein encoded by the Smad3 gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis. (provided by RefSeq)

Long Name

Mothers Against DPP Homolog 3

Alternate Names

DKFZp586N0721, DKFZp686J10186, hMAD-3, hSMAD3, HsT17436, JV15-2MGC60396, MAD homolog 3, mad protein homolog, MAD, mothers against decapentaplegic homolog 3, MAD, mothers against decapentaplegic homolog 3 (Drosophila), mad3, MADH3mad homolog JV15-2, mothers against decapentaplegic homolog 3, Mothers against DPP homolog 3, SMA- and MAD-related protein 3, SMAD 3, SMAD family member 3HSPC193, SMAD, mothers against DPP homolog 3, SMAD, mothers against DPP homolog 3 (Drosophila), Smad3

Gene Symbol

SMAD3

UniProt

P84022

Additional Smad3 Products

Product Documents for Smad3 Antibody (PCRP-SMAD3-1A2)

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Certificate of Analysis

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Product Specific Notices for Smad3 Antibody (PCRP-SMAD3-1A2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Notch Pathway

Regulatory T Cells (Tregs)

Transcription Factors in the Akt Pathway

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