Smad4 Antibody (SMAD/6309R)

Novus Biologicals | Catalog # NBP3-13963

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # SMAD/6309R
View all Smad4 Primary Antibodies »

Product Specifications

Immunogen

Recombinant human full-length (aa1-552) Smad4 protein (Uniprot: Q13485 )

Localization

Nucleus, Cytoplasm, Cytoplasmic in absence of ligand, Migrates to nucleus when complexed with R-SMAD

Marker

Pancreatic Adenocarcinoma Marker / Tumor Suppressor

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Theoretical MW

61 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14116)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for Smad4 Antibody (SMAD/6309R)

Immunohistochemistry-Paraffin: Smad4 Antibody (SMAD/6309R) [NBP3-13963]

Immunohistochemistry-Paraffin: Smad4 Antibody (SMAD/6309R) [NBP3-13963]

Immunohistochemistry-Paraffin: Smad4 Antibody (SMAD/6309R) [NBP3-13963] - Immunohistochemical stain using Smad4 antibody (SMAD/6309R) showing loss of SMAD4 expression in pancreatic ductal adenocarcinoma. Note adjacent benign ductal epithelium and background stroma as internal control showing positive / retained SMAD4 staining.
Immunohistochemistry-Paraffin: Smad4 Antibody (SMAD/6309R) [NBP3-13963]

Immunohistochemistry-Paraffin: Smad4 Antibody (SMAD/6309R) [NBP3-13963]

Immunohistochemistry-Paraffin: Smad4 Antibody (SMAD/6309R) [NBP3-13963] - Formalin-fixed, paraffin-embedded human small intestine stained with Smad4 antibody (SMAD/6309R). HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.
Smad4 Antibody (SMAD/6309R)

Smad4 Antibody (SMAD/6309R)

Formalin-fixed, paraffin-embedded human small intestine stained with Smad4 antibody (SMAD/6309R). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.

Applications for Smad4 Antibody (SMAD/6309R)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Smad4

Smad proteins, the mammalian homologs of the Drosophila Mothers against dpp (Mad) have been implicated as downstream effectors of TGFbeta/BMP signaling. Smad1 (also designated Madr1 or JV4-1), Smad5 and mammalian Smad8 (also designated Smad9 or MadH6) are effectors of BMP2 and BMP4 function while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGFbeta and activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to activin/TGFbeta signaling by interfering with TGFbeta-mediated phosphorylation of other Smad family members.

Long Name

Mothers Against DPP Homolog 4

Alternate Names

DPC4, MADH4

Gene Symbol

SMAD4

UniProt

Q13485

Additional Smad4 Products

Product Documents for Smad4 Antibody (SMAD/6309R)

Certificate of Analysis

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Product Specific Notices for Smad4 Antibody (SMAD/6309R)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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