Smad7 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-87728

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Mouse

Predicted:

Mouse (99%), Rat (99%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Smad7 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: EVKRLCCCESYGKINPELVCCNPHHLSRLCELESPPPPYSRYPMDFLKPTADCPDAVPSSAETGGTNYLAPGGLSDSQLLLEPGDRSHWCVVAYWEEKTRVGRLYCVQEPSLDIFYDLPQGNG

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit Smad7 Antibody - BSA Free (NBP1-87728) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-Smad7 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Smad7 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Smad7 Antibody [NBP1-87728]

Immunocytochemistry/ Immunofluorescence: Smad7 Antibody [NBP1-87728]

Immunocytochemistry/Immunofluorescence: Smad7 Antibody [NBP1-87728] - Staining of human cell line U-251 MG shows localization to nucleoplasm, nucleoli fibrillar center, cytosol and centrosome. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Smad7 Antibody [NBP1-87728]

Immunohistochemistry-Paraffin: Smad7 Antibody [NBP1-87728]

Immunohistochemistry-Paraffin: Smad7 Antibody [NBP1-87728] - Staining of human Liver shows very weak cytoplasmic positivity in hepatocytes.
Immunohistochemistry-Paraffin: Smad7 Antibody [NBP1-87728]

Immunohistochemistry-Paraffin: Smad7 Antibody [NBP1-87728]

Immunohistochemistry-Paraffin: Smad7 Antibody [NBP1-87728] - Staining of human lung shows nuclear positivity in pneumnocytes and macrophages.
Immunohistochemistry-Paraffin: Smad7 Antibody [NBP1-87728]

Immunohistochemistry-Paraffin: Smad7 Antibody [NBP1-87728]

Immunohistochemistry-Paraffin: Smad7 Antibody [NBP1-87728] - Staining of human Kidney shows moderate cytoplasmic positivity in cells in tubules.
Immunohistochemistry-Paraffin: Smad7 Antibody [NBP1-87728]

Immunohistochemistry-Paraffin: Smad7 Antibody [NBP1-87728]

Immunohistochemistry-Paraffin: Smad7 Antibody [NBP1-87728] - Staining of human Rectum shows moderate nuclear and cytoplasmic positivity in glandular cells.

Applications for Smad7 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50-1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Smad7

SMADs are members of the MAD-related family of molecules. MAD-related proteins are a family of intracellular proteins that are essential components in the signaling pathways of the serine/threonine kinase receptors of the transforming growth factor beta superfamily (1). SMADs can be divided into receptor-regulated SMADs (R-SMADs: SMAD1, SMAD2, SMAD5, SMAD8 and SMAD9), common-mediator SMAD (co-SMAD: SMAD4), and inhibitory SMADs (I-SMADs: SMAD6 and SMAD7). SMAD1, SMAD5, SMAD8 and SMAD9 have high degrees of homology and antibodies are available that recognize sequences common to all of them. SMAD8 and SMAD9 are typically used asalternate namesfor one another in the literature. Human SMAD1 is a 465 amino acid protein; GenBank Accession No. AAP36050.1. Human SMAD2 is a 467 amino acid protein; GenBank Accession No. AAC51918.1 Human SMAD3 is a 425 amino acid protein; GenBank Accession No. NP_005893.1 Human SMAD4 is a 552 amino acid protein GenBank Accession No. NP_005350.1. Human SMAD5 is a 465 amino acid protein; GenBank Accession No. AAC50791.1. Human SMAD6 is a 496 amino acid protein; GenBank Accession No. AAH12986.1 Human SMAD7 is a 426 amino acid protein; GenBank Accession No. AAB81354.1 Mouse SMAD8 is a 430 amino acid protein; GenBank Accession No. AAN85445.1 Human SMAD9 is a 430 amino acid protein; GenBank Accession No. NP_005896.1

Long Name

Mothers Against DPP Homolog 7

Alternate Names

MADH7, MADH8

Gene Symbol

SMAD7

Additional Smad7 Products

Product Documents for Smad7 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Smad7 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Smad7 Antibody - BSA Free

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Protocols

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FAQs

No product specific FAQs exist for this product.

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Associated Pathways

TGF-beta Signaling Pathways TGF-beta Signaling Pathway Thumbnail