Smad9 Antibody (PCRP-SMAD9-2F4)
Novus Biologicals | Catalog # NBP3-13964
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Protein Array
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # PCRP-SMAD9-2F4
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Product Specifications
Immunogen
Recombinant full-length human Smad9 protein (Uniprot: O15198)
Localization
Cytoplasm, Nucleus. In the cytoplasm in the absence of ligand, Migration to the nucleus when complexed with SMAD4
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
52.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14117)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for Smad9 Antibody (PCRP-SMAD9-2F4)
![Flow Cytometry: Smad9 Antibody (PCRP-SMAD9-2F4) [NBP3-13964]](https://resources.rndsystems.com/images/products/Smad9-Antibody-PCRP-SMAD9-2F4-Flow-Cytometry-NBP3-13964-img0001.jpg "Flow Cytometry: Smad9 Antibody (PCRP-SMAD9-2F4) [NBP3-13964]")
Flow Cytometry: Smad9 Antibody (PCRP-SMAD9-2F4) [NBP3-13964]
Flow Cytometry: Smad9 Antibody (PCRP-SMAD9-2F4) [NBP3-13964] - Flow cytometric analysis of PFA-fixed HeLa cells. Smad9 antibody (PCRP-SMAD9-2F4) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).![Protein Array: Smad9 Antibody (PCRP-SMAD9-2F4) [NBP3-13964]](https://resources.rndsystems.com/images/products/Smad9-Antibody-PCRP-SMAD9-2F4-Protein-Array-NBP3-13964-img0004.jpg "Protein Array: Smad9 Antibody (PCRP-SMAD9-2F4) [NBP3-13964]")
Protein Array: Smad9 Antibody (PCRP-SMAD9-2F4) [NBP3-13964]
Protein Array: Smad9 Antibody (PCRP-SMAD9-2F4) [NBP3-13964] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Smad9 Antibody (PCRP-SMAD9-2F4).![Smad9 Antibody (PCRP-SMAD9-2F4) Immunocytochemistry/ Immunofluorescence: Smad9 Antibody (PCRP-SMAD9-2F4) [NBP3-13964] -](https://resources.rndsystems.com/images/products/nbp3-13964_mouse-smad9-mab-pcrp-smad9-2f4-29520251636949.jpg "Immunocytochemistry/ Immunofluorescence: Smad9 Antibody (PCRP-SMAD9-2F4) [NBP3-13964] -")
Immunocytochemistry/ Immunofluorescence: Smad9 Antibody (PCRP-SMAD9-2F4) [NBP3-13964] -
Immunofluorescence Analysis of PFA-fixed HeLa cells stained using Smad9 Antibody (PCRP-SMAD9-2F4) followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).![Smad9 Antibody (PCRP-SMAD9-2F4) Immunocytochemistry/ Immunofluorescence: Smad9 Antibody (PCRP-SMAD9-2F4) [NBP3-13964] -](https://resources.rndsystems.com/images/products/nbp3-13964_mouse-smad9-mab-pcrp-smad9-2f4-29520251641498.jpg "Immunocytochemistry/ Immunofluorescence: Smad9 Antibody (PCRP-SMAD9-2F4) [NBP3-13964] -")
Immunocytochemistry/ Immunofluorescence: Smad9 Antibody (PCRP-SMAD9-2F4) [NBP3-13964] -
Immunofluorescence Analysis of PFA-fixed HeLa cells stained using Smad9 Antibody (PCRP-SMAD9-2F4)Applications for Smad9 Antibody (PCRP-SMAD9-2F4)
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Smad9
Long Name
Mothers Against DPP Homolog 9
Alternate Names
Madh6, MADH9
Gene Symbol
SMAD9
UniProt
Additional Smad9 Products
Product Documents for Smad9 Antibody (PCRP-SMAD9-2F4)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Smad9 Antibody (PCRP-SMAD9-2F4)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Liperfluo
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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