SMARCA5/SNF2H Antibody (JE40-44)

Novus Biologicals | Catalog # NBP3-32963

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JE40-44 expressed in HEK293
View all SMARCA5/SNF2H Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide within Human SMARCA5/SNF2H aa 1,003-1,052 / 1,052. (Uniprot: O60264)

Localization

Nucleus, Chromosome.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

122 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SMARCA5/SNF2H Antibody (JE40-44)

SMARCA5/SNF2H Antibody (JE40-44)

Western Blot: SMARCA5/SNF2H Antibody (JE40-44) [NBP3-32963]

Western Blot: SMARCA5/SNF2H Antibody (JE40-44) [NBP3-32963] - Western blot analysis of SMARCA5/SNF2H on different lysates with Rabbit anti-SMARCA5/SNF2H antibody (NBP3-32963) at 1/1,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: U-2 OS cell lysate
Lane 3: Jurkat cell lysate
Lane 4: HepG2 cell lysate
Lane 5: A549 cell lysate
Lane 6: NIH/3T3 cell lysate
Lane 7: PC-12 cell lysate

Lysates/proteins at 20 ug/Lane.

Predicted band size: 122 kDa
Observed band size: 122 kDa

Exposure time: 1 minute 55 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32963) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.

Applications for SMARCA5/SNF2H Antibody (JE40-44)

Application
Recommended Usage

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

1*TBS (pH7.4), 0.05% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SMARCA5/SNF2H

The SWI/SNF-related, matrix-associated, actin-dependent regulators of chromatin (SMARC), also called BRG1-associated factors (BAFs), have been identified as components of the human SWI/SNF-like chromatin-remodeling protein complexes. SNF2h/ISWI, also known as WCRF135, is the gene product of SMARCA5 that associates with WSTF (Williams Syndrome transcription factor) and several other nuclear proteins to mediate chromatin remodeling. SNF2h is also part of the NoRC (nucleolar remodeling complex).

Long Name

SWI/SNF-related, Matrix-associated, Actin-dependent Regulator of Chromatin, Subfamily A, Member 5

Alternate Names

ISWI, SNF2H, WCRF135

Gene Symbol

SMARCA5

Additional SMARCA5/SNF2H Products

Product Documents for SMARCA5/SNF2H Antibody (JE40-44)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for SMARCA5/SNF2H Antibody (JE40-44)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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