SMC1 Antibody (BL-205-2G8)

Novus Biologicals | Catalog # NBP2-76412

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # BL-205-2G8
View all SMC1 Primary Antibodies »

Product Specifications

Immunogen

Immunogen sequence between amino acids 1200 and 1233 (C-Term)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SMC1 Antibody (BL-205-2G8)

Western Blot: SMC1 Antibody (BL-205-2G8) [NBP2-76412]

Western Blot: SMC1 Antibody (BL-205-2G8) [NBP2-76412]

Western Blot: SMC1 Antibody (BL-205-2G8) [NBP2-76412] - Detection of human and mouse SMC1 by WB of HeLa, HEK293T, MCF-7, Hep-G2, A-549, SW620, SK-MEL-28 (SK), Jurkat, TCMK-1, NIH 3T3, and CT26 lysate. Antibody: Rabbit anti-SMC1 recombinant monoclonal [BL-205-2G8] (NBP2-76412). Secondary: HRP-conjugated goat anti-rabbit IgG.
Immunocytochemistry: SMC1 Antibody (BL-205-2G8) [NBP2-76412]

Immunocytochemistry: SMC1 Antibody (BL-205-2G8) [NBP2-76412]

Immunocytochemistry: SMC1 Antibody (BL-205-2G8) [NBP2-76412] - Detection of human SMC1 in FFPE HCT 116 cells by ICC. Antibody: Rabbit anti-SMC1 recombinant monoclonal [BL-205-2G8] (NBP2-76412). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
Immunohistochemistry: SMC1 Antibody (BL-205-2G8) [NBP2-76412]

Immunohistochemistry: SMC1 Antibody (BL-205-2G8) [NBP2-76412]

Immunohistochemistry: SMC1 Antibody (BL-205-2G8) [NBP2-76412] - Detection of human SMC1 in FFPE breast carcinoma by IHC. Antibody: Rabbit anti-SMC1 recombinant monoclonal [BL-205-2G8] (NBP2-76412). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
Immunoprecipitation: SMC1 Antibody (BL-205-2G8) [NBP2-76412]

Immunoprecipitation: SMC1 Antibody (BL-205-2G8) [NBP2-76412]

Immunoprecipitation: SMC1 Antibody (BL-205-2G8) [NBP2-76412] - Detection of human SMC1 by WB of immunoprecipitates from HEK293T lysate. Antibodies: Rabbit anti-SMC1 recombinant monoclonal {BL-205-2G8] (NBP2-76412) and rabbit anti-SMC1 (NB100-204).

Applications for SMC1 Antibody (BL-205-2G8)

Application
Recommended Usage

Immunoprecipitation

20 ul/mg lysate

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 2 - 8 C / 1 year from date of receipt

Background: SMC1

Structural maintenance of chromosomes (SMC) proteins are part of the cohesin and condensin multisubunit complexes that play a critical role in chromosome organization, segregation, gene regulation, and DNA repair. The core cohesin complex includes a heterodimer of SMC1 and SMC3 that functions by forming a ring-like structure that holds sister chromatids together. Cohesin is important to sister chromatid separation and segregation during mitosis and meiosis and also plays a role in double-strand break repair by homologous recombination.

Long Name

Structural Maintenance of Chromosomes 1

Alternate Names

SB1.8, SMC1A, SMC1L1

Gene Symbol

SMC1A

Additional SMC1 Products

Product Documents for SMC1 Antibody (BL-205-2G8)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for SMC1 Antibody (BL-205-2G8)

This Product sold under License from Bethyl Laboratories, Inc.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for SMC1 Antibody (BL-205-2G8)

Showing  1 - 2 of 2 FAQs Showing All

  • Q: I would like to use one of your anti-smc1 antibodies to detect smc1 in an invertebrate. Your different antibodies are raised against different peptides derived from different regions possibly well-conserved, but not completely, in the organism that interests me (Schistosoma mansoni). Would it be possible to know the sequence of the different peptides that was used to generate the different antibodies, or failing that, could you analyze the corresponding sequence in my species of interest in order to determine whether or not it corresponds to one of the different peptides?

    A: My suggestion to you would be to look at the homology between the protein expressed in your species and the protein expressed in the reactive species of the antibodies. A homology of >90% is a great start, >80% is acceptable. Please view our list of smc1 antibodies we currently offer and there are 27 products.

  • Q: My sequence of interest have >80% homology with the N-term and the C-term parts of human smc1. Your antibodies NB100-78322 and NB100-204 recognized the N-term and C-term of human smc1 respectively, therefore it’s possible that the cross reactivity can happen. I would be very interested to test these antibodies (NB100-78322 and NB100-204). Can you provide me free sample (few ul) to do western blot. I will share the testing result with you.

    A: We do not provide free samples for testing. If you are interested in testing these antibodies in an unlisted species or application, you would qualify for the Innovator's Rewards program. The way the program works is that you would purchase the antibody of interest, perform your testing using appropriate controls and then share your data with us. Upon review of your data we would issue you a voucher for a free antibody. You can find more details here.

  • Q: I would like to use one of your anti-smc1 antibodies to detect smc1 in an invertebrate. Your different antibodies are raised against different peptides derived from different regions possibly well-conserved, but not completely, in the organism that interests me (Schistosoma mansoni). Would it be possible to know the sequence of the different peptides that was used to generate the different antibodies, or failing that, could you analyze the corresponding sequence in my species of interest in order to determine whether or not it corresponds to one of the different peptides?

    A: My suggestion to you would be to look at the homology between the protein expressed in your species and the protein expressed in the reactive species of the antibodies. A homology of >90% is a great start, >80% is acceptable. Please view our list of smc1 antibodies we currently offer and there are 27 products.

  • Q: My sequence of interest have >80% homology with the N-term and the C-term parts of human smc1. Your antibodies NB100-78322 and NB100-204 recognized the N-term and C-term of human smc1 respectively, therefore it’s possible that the cross reactivity can happen. I would be very interested to test these antibodies (NB100-78322 and NB100-204). Can you provide me free sample (few ul) to do western blot. I will share the testing result with you.

    A: We do not provide free samples for testing. If you are interested in testing these antibodies in an unlisted species or application, you would qualify for the Innovator's Rewards program. The way the program works is that you would purchase the antibody of interest, perform your testing using appropriate controls and then share your data with us. Upon review of your data we would issue you a voucher for a free antibody. You can find more details here.

Showing  1 - 2 of 2 FAQs Showing All
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