SMG1 Antibody (5N10C7)

Novus Biologicals | Catalog # NBP3-15716

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 5N10C7 expressed in HEK293
View all SMG1 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 3562-3661 of human SMG1 (Q96Q15). LIQKNLATSADTPPSTVPGTGKSVACSPKKAVRDPKTGKAVQERNSYAVSVWKRVKAKLEGRDVDPNRRMSVAEQVDYVIKEATNLDNLAQLYEGWTAWV

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SMG1 Antibody (5N10C7)

Western Blot: SMG1 Antibody (5N10C7) [NBP3-15716]

Western Blot: SMG1 Antibody (5N10C7) [NBP3-15716]

Western Blot: SMG1 Antibody (5N10C7) [NBP3-15716] - Western blot analysis of extracts of various cell lines, using SMG1 antibody (NBP3-15716) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Applications for SMG1 Antibody (5N10C7)

Application
Recommended Usage

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SMG1

SMG1 encodes a protein involved in nonsense-mediated mRNA decay (NMD) as part of the mRNA surveillance complex. The protein has kinase activity and is thought to function in NMD by phosphorylating the regulator of nonsense transcripts 1 protein. Alternative spliced transcript variants have been described, but their full-length natures have not been determined.

Alternate Names

ATXserine/threonine-protein kinase SMG1, EC 2.7.11, EC 2.7.11.1, hSMG-1,61E3.4, KIAA0421phosphatidylinositol 3-kinase-related protein kinase, Lambda/iota protein kinase C-interacting protein, Lambda-interacting protein, LIPPI-3-kinase-related kinase SMG-1, phosphatidylinositol 3-kinase-related kinase, SMG-1, SMG1 homolog, phosphatidylinositol 3-kinase-related kinase (C. elegans)

Gene Symbol

SMG1

Additional SMG1 Products

Product Documents for SMG1 Antibody (5N10C7)

Certificate of Analysis

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Product Specific Notices for SMG1 Antibody (5N10C7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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