SNAP25 Antibody

Novus Biologicals | Catalog # NB100-1492

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse, Rat

Predicted:

Canine (100%). Backed by our 100% Guarantee.

Applications

Validated:

Western Blot, Peptide ELISA

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all SNAP25 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-DEANQRATKMLGSG corresponding to C-Terminus according to NP_003072.2, NP_570824.1.

Specificity

This antibody is expected to recognise both reported isoforms NP_003072.2 (SNAP25A) and NP_570824.1 (SNAP25B). Reported variants represent identical protein: NP_001309831.1, NP_003072.2 Reported variants represent identical protein: NP_001309834.1, NP_001309832.1, NP_001309833.1, NP_001309839.1, NP_001309836.1, NP_570824.1, NP_001309838.1, NP_001309835.1, NP_001309837.1

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for SNAP25 Antibody

Western Blot: SNAP25 Antibody [NB100-1492]

Western Blot: SNAP25 Antibody [NB100-1492]

Western Blot: SNAP25 Antibody [NB100-1492] - (0.005ug/ml) staining of Human Brain (Cerebellum) (A), Mouse Brain (B) and Rat Brain (C) lysates (35ug protein in RIPA buffer). Detected by chemiluminescence.
SNAP25 Antibody

Western Blot: SNAP25 Antibody [NB100-1492] -

Extraction and characterization of synaptosomes.a Brief workflow of the current study. b Immunoblotting analysis of synaptic (SNAP25, synaptophysin and PSD95) and cytosolic (elF1a and PCNA) proteins in cytosolic fraction, synaptosomal fraction and leftover tissue debris of unaffected control postmortem brain tissues. c Densitometry analysis of synaptic and cytosolic proteins. Synaptic proteins levels (PSD95; P = 0.003), (SNAP25; P = 0.0061), (Synaptophysin; P = 0.026) were significantly higher in synaptosomes and cytosolic proteins (elF1a; P = 0.012) and (PCNA; P = 0.018) levels were significantly lower in synaptosomes relative to cytosol. d qRT-PCR analysis for mRNA fold change analysis of synaptic and cytosolic genes in cytosolic and synaptosomal fractions (n = 5). e TEM analysis of synapse assembly in synaptosomal fraction from unaffected control and AD patients’ postmortem brains (scale bar 500 nm magnification). Electron micrograph shows synapse components: Mt mitochondria, SV synaptic vesicles, PSD postsynaptic density, SC synaptic cleft. f Immunoblotting analysis of brain cells markers (Neuron-NeuN; Microglia-Iba1), excitatory synapse marker (VGLUT1) and inhibitory synapse marker (GABARA1) proteins in unaffected controls (n = 4) and AD (n = 4) synaptosomes. g Densitometry analysis of NeuN, Iba1, VGLUT1, and GABARA1 proteins in unaffected controls and AD synaptosomes. All blots are driven from the same experiment and were proceed parallelly (b, f). Values in the bar diagrams are mean +/- SEM and error bars are equivalent throughout the figure (c, d, g). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35941185), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for SNAP25 Antibody

Application
Recommended Usage

Peptide ELISA

detection limit 1:64000

Western Blot

0.005-0.01ug/ml
Application Notes
Western blot: Approx 26 kDa band observed in Human Brain and Mouse and Rat Brain lysates (calculated MW of 23.3kDa according to Human NP_003072.2, Mouse NP_035558.1 and Rat NP_001257504.1).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SNAP25

SNAP 25 (synaptosome-associated protein of 25,000 dalton) is a highly conserved protein anchored to the cytosolic face of membranes via palmitoyl side chains in the middle of the molecule. SNAP 25 is the target of Botulinum neurotoxin A and E which cleave off 9 and 26 amino acids, respectively, from the C-terminus. The protein is part of the exocytotic fusion complex (v-SNARE) of neurons where it assembles with syntaxin 1 and synaptobrevin/VAMP. The protein is abundantly localized on the neuronal plasmalemma and on recycling vesicles including synaptic vesicles. It is also expressed in neuroendocrine cells.

Long Name

Synaptosomal-associated Protein, 25 kDa

Alternate Names

RIC4, SEC9, SUP

Entrez Gene IDs

6616 (Human); 20614 (Mouse)

Gene Symbol

SNAP25

UniProt

NP_003072.2 (Human)

Additional SNAP25 Products

Product Documents for SNAP25 Antibody

Certificate of Analysis

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Product Specific Notices for SNAP25 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Neuronal Markers

Citations for SNAP25 Antibody

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