SOCS-3 Antibody (SO1) - BSA Free

Novus Biologicals | Catalog # NB600-1075

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Cited:

Rat

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # SO1

Format

BSA Free
View all SOCS-3 Primary Antibodies »

Product Specifications

Immunogen

Full-length SOCS3 protein.

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 24142708).

Specificity

The antibody SO1 reacts with SOCS3, an intracellular cytokine signaling inhibitor.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for SOCS-3 Antibody (SO1) - BSA Free

Western Blot: SOCS-3 Antibody (SO1)BSA Free [NB600-1075]

Western Blot: SOCS-3 Antibody (SO1)BSA Free [NB600-1075]

Western Blot: SOCS3 Antibody (SO1) [NB600-1075] - Fig. 1. Western Blotting analysis (reducing conditions) of whole cell lysate of HeLa human cervix carcinoma cell line. Lane 1: immunostaining with Isotype mouse IgG2b control (PLRV219) Lane 2: immunostaining with anti-SOCS3 (SO1).

Applications for SOCS-3 Antibody (SO1) - BSA Free

Application
Recommended Usage

Western Blot

1:1000
Application Notes
Western Blot - Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with reducing Laemmli SDS-PAGE sample buffer. Application note: Reducing conditions.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

Phosphate buffered saline (PBS), pH 7.4

Format

BSA Free

Preservative

15mM Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: SOCS-3

SOCS3 (suppressor of cytokine signaling 3), also known as CIS3 (cytokine-inducible SH2 protein 3) is a negative regulator of particular cytokine signaling pathways. SOCS3 is induced by a variety of cytokines and other stimuli, such as erythropoietin, leptin and lipopolysaccharides and inhibits tyrosinkinase activity of JAK kinases, or e.g. JNK phosphorylation. SOCS3 modulates cytokine-mediated and neoplastic-proliferative responses and is involved also in maintaining leukocytes in quiescent state until antigen stimulation.

Long Name

Suppressor Of Cytokine Signaling 3

Alternate Names

ATOD4, CIS3, Cish3, SOCS3, SSI-3

Entrez Gene IDs

89829 (Rat)

Gene Symbol

SOCS3

Additional SOCS-3 Products

Product Documents for SOCS-3 Antibody (SO1) - BSA Free

Certificate of Analysis

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Product Specific Notices for SOCS-3 Antibody (SO1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for SOCS-3 Antibody (SO1) - BSA Free

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