SODD/BAG4 Antibody - BSA Free

Novus Biologicals | Catalog # NB100-56696

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Flow Cytometry, Flow (Cell Surface), Knockdown Validated

Cited:

Western Blot, Flow (Cell Surface)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all SODD/BAG4 Primary Antibodies »

Product Specifications

Immunogen

This polyclonal antibody was raised against a peptide corresponding to amino acids 443-457 of human SODD protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SODD/BAG4 Antibody - BSA Free

Knockdown Validated: SODD/BAG4 Antibody [NB100-56696]

Western Blot: SODD/BAG4 Antibody [NB100-56696]

Western Blot: SODD/BAG4 Antibody [NB100-56696] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and SODD/BAG4 knockout (KO) HeLa cell line. PVDF membrane was probed with 1 ug/ml of Rabbit Anti-Human SODD/BAG4 Polyclonal Antibody (Catalog # NB100-56696) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for SODD/BAG4 at approximately 75 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.
Western Blot: SODD/BAG4 Antibody [NB100-56696]

Western Blot: SODD/BAG4 Antibody [NB100-56696]

Western Blot: SODD/BAG4 Antibody [NB100-56696] - Analysis of 30 ug of total cell lysate from HeLa cells with anti-SODD at 2 ug/ml.

Applications for SODD/BAG4 Antibody - BSA Free

Application
Recommended Usage

Flow (Cell Surface)

reported in scientific literature (PMID 15592361)

Flow Cytometry

1:10-1:1000

Western Blot

2-4 ug/ml

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS containing 0.2% gelatin

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SODD/BAG4

Several cell surface receptors that are involved in apoptosis contain intracellular death domains and are capable of triggering apoptosis when activated by their respective ligands (1). Due to presence of these death domains and presence of various stimuli in the serum as well as overexpression leading to receptor oligomerization, which leads to constitutive activation of the apoptotic pathway, it has been extremely difficult to generate permanent cell lines overexpressing specific receptors in culture. However, in normal states, this death domain activity is silenced presumably due to presence of other cellular factors. Recently, a DR3 associated protein has been isolated by yeast two-hybrid interaction assays. This cDNA encoding a 457-amino acid protein has been named as SODD (Silencer of Death Domain) (2). SODD also associates with the intracellular domains of TNF-R1, but not with those of TNF-R2, Fas, DR4, or DR5. SODD overexpression suppressed the TNF induced cell death as well as NF-kB activation (2). SODD may act as a negative regulatory protein that is normally associated with death domain of TNF-R1 and probably inhibit self-oligomerization properties of death domain and maintain TNF-R1 in an inactive, monomeric state.

Long Name

Silencer of Death Domains

Alternate Names

BAG4

Gene Symbol

BAG4

UniProt

O95429

Additional SODD/BAG4 Products

Product Documents for SODD/BAG4 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for SODD/BAG4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Apoptosis Adaptor Proteins

Citations for SODD/BAG4 Antibody - BSA Free

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FAQs

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Associated Pathways

Apoptosis Signaling Pathway Apoptosis Signaling Pathway Thumbnail