Human Sorbitol Dehydrogenase ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP3-40082

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, tissue homogenates and other biological fluids (100 uL)

Sensitivity

1.25 ng/mL (example only; lot dependent)

Assay Range

3.125 - 200 ng/mL (example only; lot dependent)
View all Sorbitol Dehydrogenase ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Human

Description

Assay Length: 4.5 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV 10 (example only; lot dependent)

Inter-Assay Precision (Precision between assays) %CV 12 (example only; lot dependent)

Scientific Data Images for Human Sorbitol Dehydrogenase ELISA Kit (Colorimetric)

Human Sorbitol Dehydrogenase ELISA Kit (Colorimetric)

ELISA: Human Sorbitol Dehydrogenase ELISA Kit (Colorimetric) [NBP3-40082]

ELISA: Human Sorbitol Dehydrogenase ELISA Kit (Colorimetric) [NBP3-40082] - Standard Curve Reference

Kit Contents for Human Sorbitol Dehydrogenase ELISA Kit (Colorimetric)

  • Detection Reagent A
  • Detection Reagent B
  • Diluent Buffer
  • Instruction manual
  • Plate sealer for 96 wells
  • Pre-coated 96T strip plate
  • Standard
  • Stop Solution
  • TMB Substrate
  • Wash Buffer (30 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Sorbitol Dehydrogenase

Sorbitol dehydrogenase (SDH), a member of the medium-chain dehydrogenase/reductase protein family and the second enzyme of the polyol pathway of glucose metabolism, converts sorbitol to fructose strictly using NAD(+) as coenzyme. SDH is expressed almost ubiquitously in all mammalian tissues. The enzyme has attracted considerable interest due to its implication in the development of diabetic complications as the polyol pathway is particularly active in hyperglycemic states. Although SORD is closely related to the class I long-chain alcohol dehydrogenases, it differs in substrate specificity, catalyzing polyols such as sorbitol and xylitol but having no activity towards primary alcohols.

Alternate Names

EC 1.1.1, EC 1.1.1.14, L-iditol 2-dehydrogenase, sorbitol dehydrogenase, SORD1

Gene Symbol

SORD

Additional Sorbitol Dehydrogenase Products

Product Documents for Human Sorbitol Dehydrogenase ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Human Sorbitol Dehydrogenase ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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