SOX4 Antibody (CL5634) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-44187

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # CL5634

Format

Azide and BSA Free
View all SOX4 Primary Antibodies »

Product Specifications

Immunogen

This antibody was generated using a synthetic peptide of Q06945, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for SOX4 Antibody (CL5634) - Azide and BSA Free

SOX4 Antibody (CL5634) - Azide and BSA Free Immunohistochemistry-Paraffin: SOX4 Antibody [NBP3-44187]

Immunohistochemistry-Paraffin: SOX4 Antibody [NBP3-44187]

Staining of human liver shows no nuclear positivity in hepatocytes as expected.
SOX4 Antibody (CL5634) - Azide and BSA Free Immunohistochemistry-Paraffin: SOX4 Antibody [NBP3-44187]

Immunohistochemistry-Paraffin: SOX4 Antibody [NBP3-44187]

Staining of human glioma shows moderate to strong nuclear positivity in tumor cells.
SOX4 Antibody (CL5634) - Azide and BSA Free Immunohistochemistry-Paraffin: SOX4 Antibody [NBP3-44187]

Immunohistochemistry-Paraffin: SOX4 Antibody [NBP3-44187]

Staining of human endometrium shows moderate to strong nuclear positivity in stromal cells.
SOX4 Antibody (CL5634) - Azide and BSA Free Immunohistochemistry-Paraffin: SOX4 Antibody [NBP3-44187]

Immunohistochemistry-Paraffin: SOX4 Antibody [NBP3-44187]

Staining of rat dentate gyrus shows strong nuclear positivity in a subset of cells in granular layer.
SOX4 Antibody (CL5634) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: SOX4 Antibody [NBP3-44187]

Immunocytochemistry/ Immunofluorescence: SOX4 Antibody [NBP3-44187]

Staining of SH-SY5Y cells using the Anti-SOX4 monoclonal antibody).
SOX4 Antibody (CL5634) - Azide and BSA Free Immunohistochemistry-Paraffin: SOX4 Antibody [NBP3-44187]

Immunohistochemistry-Paraffin: SOX4 Antibody [NBP3-44187]

Staining of mouse brain shows strong nuclear positivity in rostral migratory stream cells.
SOX4 Antibody (CL5634) - Azide and BSA Free Immunohistochemistry-Paraffin: SOX4 Antibody [NBP3-44187]

Immunohistochemistry-Paraffin: SOX4 Antibody [NBP3-44187]

Staining of mouse dentate gyrus shows strong nuclear positivity in a subset of neurons in the granular cell layer.

Applications for SOX4 Antibody (CL5634) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry-Paraffin

1:1000 - 1:2500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/ Immunofluorescence/IF Fixation Permeabilization: PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SOX4

SOX4 encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional regulator after forming a protein complex with other proteins, such as syndecan binding protein (syntenin). The protein may function in the apoptosis pathway leading to cell death as well as to tumorigenesis and may mediate downstream effects of parathyroid hormone (PTH) and PTH-related protein (PTHrP) in bone development. The solution structure has been resolved for the HMG-box of a similar mouse protein.

Alternate Names

ecotropic viral integration site 16, EVI16, SRY (sex determining region Y)-box 4, SRY-related HMG-box gene 4, transcription factor SOX-4

Gene Symbol

SOX4

Additional SOX4 Products

Product Documents for SOX4 Antibody (CL5634) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for SOX4 Antibody (CL5634) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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