SPAG1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-41374

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit

Format

BSA Free
View all SPAG1 Primary Antibodies »

Product Specifications

Immunogen

Immunized with recombinant Human SPAG1 (Uniprot#: Q07617; Met1-Lys365 (Uniprot#: Q07617)

Clonality

Polyclonal

Host

Rabbit

Scientific Data Images for SPAG1 Antibody - BSA Free

SPAG1 Antibody

Western Blot: SPAG1 Antibody [NBP3-41374] -

Western Blot: SPAG1 Antibody [NBP3-41374] - Sample: Human Jurkat cell lysate; Primary Ab: 1ug/ml NBP3-41374; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
SPAG1 Antibody

Immunohistochemistry-Paraffin: SPAG1 Antibody [NBP3-41374] -

Immunohistochemistry-Paraffin: SPAG1 Antibody [NBP3-41374] - DAB staining on IHC-P; Samples: Human Liver Tissue; Primary Ab: 10ug/ml NBP3-41374; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
SPAG1 Antibody

Western Blot: SPAG1 Antibody [NBP3-41374] -

Western Blot: SPAG1 Antibody [NBP3-41374] - Sample: Recombinant protein.

Applications for SPAG1 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

5-20ug/mL

Immunohistochemistry-Paraffin

5-20ug/mL

Western Blot

0.2-2ug/mL

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS, pH7.4, 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SPAG1

The correlation of anti-sperm antibodies with cases of unexplained infertility implicates a role for these antibodies in blocking fertilization. Improved diagnosis and treatment of immunologic infertility, as well as identification of proteins for targeted contraception, are dependent on the identification and characterization of relevant sperm antigens. The protein expressed by this gene is recognized by anti-sperm agglutinating antibodies from an infertile woman. Furthermore, immunization of female rats with the recombinant human protein reduced fertility. This protein localizes to the plasma membrane of germ cells in the testis and to the post-acrosomal plasma membrane of mature spermatozoa. Recombinant polypeptide binds GTP and exhibits GTPase activity. Thus, this protein may regulate GTP signal transduction pathways involved in spermatogenesis and fertilization. Two transcript variants of this gene encode the same protein.

Alternate Names

FLJ32920, HSD-3.8sperm-associated antigen 1, Infertility-related sperm protein Spag-1, SP75, sperm associated antigen 1, tetratricopeptide repeat-containing protein, TPIS, TPR-containing protein involved in spermatogenesis

Gene Symbol

SPAG1

Additional SPAG1 Products

Product Documents for SPAG1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for SPAG1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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