Rat SPARC ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP2-76783

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

18.75pg/mL

Assay Range

31.25 - 2000pg/mL
View all SPARC ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Rat

Precision

Intra-Assay Precision (Precision within an assay) CV% 4.67

Inter-Assay Precision (Precision between assays) CV% 4.68

Recovery for Rat SPARC ELISA Kit (Colorimetric)

Recovery

84-105%

Linearity

Rat SPARC ELISA Kit (Colorimetric)

Scientific Data Images for Rat SPARC ELISA Kit (Colorimetric)

Rat SPARC ELISA Kit (Colorimetric)

ELISA: Rat SPARC ELISA Kit (Colorimetric) [NBP2-76783] -

ELISA: Rat SPARC ELISA Kit (Colorimetric) [NBP2-76783] - Standard Curve Reference

Kit Contents for Rat SPARC ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: SPARC

Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity.

Long Name

Secreted Protein Acidic and Rich in Cysteine

Alternate Names

BM-40, Osteonectin

Gene Symbol

SPARC

Additional SPARC Products

Product Documents for Rat SPARC ELISA Kit (Colorimetric)

Certificate of Analysis

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Product Specific Notices for Rat SPARC ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Citations for Rat SPARC ELISA Kit (Colorimetric)

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