SPATA18 Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-81984
![Immunohistochemistry-Paraffin: SPATA18 Antibody [NBP1-81984]](https://resources.rndsystems.com/images/products/SPATA18-Antibody-Immunohistochemistry-Paraffin-NBP1-81984-img0020.jpg "Immunohistochemistry-Paraffin: SPATA18 Antibody [NBP1-81984]")
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Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: AENLKRLVSNETLRTLQEKLDFWLKEYNTNTCDQNLNHCLELIEQVAKVQGQLFGILTAAAQEGGRNDGVETIKSR
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
61 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for SPATA18 Antibody - BSA Free
Immunohistochemistry-Paraffin: SPATA18 Antibody [NBP1-81984]
Immunohistochemistry-Paraffin: SPATA18 Antibody [NBP1-81984] - Analysis in human fallopian tube and prostate tissues using NBP1-81984 antibody. Corresponding SPATA18 RNA-seq data are presented for the same tissues.![Immunohistochemistry-Paraffin: SPATA18 Antibody [NBP1-81984]](https://resources.rndsystems.com/images/products/SPATA18-Antibody-Immunohistochemistry-Paraffin-NBP1-81984-img0016.jpg "Immunohistochemistry-Paraffin: SPATA18 Antibody [NBP1-81984]")
Immunohistochemistry-Paraffin: SPATA18 Antibody [NBP1-81984]
Immunohistochemistry-Paraffin: SPATA18 Antibody [NBP1-81984] - Staining of human fallopian tube shows strong positivity in cilia in glandular cells.![Immunohistochemistry-Paraffin: SPATA18 Antibody [NBP1-81984]](https://resources.rndsystems.com/images/products/SPATA18-Antibody-Immunohistochemistry-Paraffin-NBP1-81984-img0017.jpg "Immunohistochemistry-Paraffin: SPATA18 Antibody [NBP1-81984]")
Immunohistochemistry-Paraffin: SPATA18 Antibody [NBP1-81984]
Immunohistochemistry-Paraffin: SPATA18 Antibody [NBP1-81984] - Staining of human lymph node shows no positivity in non-germinal center cells as expected.![Immunohistochemistry-Paraffin: SPATA18 Antibody [NBP1-81984]](https://resources.rndsystems.com/images/products/SPATA18-Antibody-Immunohistochemistry-Paraffin-NBP1-81984-img0018.jpg "Immunohistochemistry-Paraffin: SPATA18 Antibody [NBP1-81984]")
Immunohistochemistry-Paraffin: SPATA18 Antibody [NBP1-81984]
Immunohistochemistry-Paraffin: SPATA18 Antibody [NBP1-81984] - Staining of human prostate shows no positivity in glandular cells as expected.![Immunohistochemistry-Paraffin: SPATA18 Antibody [NBP1-81984]](https://resources.rndsystems.com/images/products/SPATA18-Antibody-Immunohistochemistry-Paraffin-NBP1-81984-img0019.jpg "Immunohistochemistry-Paraffin: SPATA18 Antibody [NBP1-81984]")
Immunohistochemistry-Paraffin: SPATA18 Antibody [NBP1-81984]
Immunohistochemistry-Paraffin: SPATA18 Antibody [NBP1-81984] - Staining of human skin shows no positivity in squamous epithelial cells as expected.
Immunohistochemistry-Paraffin: SPATA18 Antibody [NBP1-81984] -
Immunohistochemistry-Paraffin: SPATA18 Antibody [NBP1-81984] - Staining of human fallopian tube, lymph node, prostate and skin using Anti-SPATA18 antibody NBP1-81984 (A) shows similar protein distribution across tissues to independent antibody (B).Applications for SPATA18 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: SPATA18
Alternate Names
FLJ32906, Mieap, mitochondria-eating protein, spermatogenesis associated 18 homolog (rat), Spermatogenesis-associated protein 18, SPETEX1
Entrez Gene IDs
132671 (Human)
Gene Symbol
SPATA18
UniProt
Additional SPATA18 Products
Product Documents for SPATA18 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for SPATA18 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for SPATA18 Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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