SPATS2 Antibody - BSA Free
Novus Biologicals | Catalog # NBP3-29150
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Key Product Details
Species Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
The epitope recognized by NBP3-29150 maps to a region between residue 495 to 545 of human Spermatogenesis-associated serine-rich protein 2 using the numbering given in entry Q86XZ4.1 (GeneID 65244).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for SPATS2 Antibody - BSA Free
Immunoprecipitation:SPATS2 AntibodyNBP3-29150] -
Immunoprecipitation:SPATS2 AntibodyNBP3-29150] -Detection of human SPATS2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SPATS2 antibody NBP3-29150 (lot NBP3-29150-1) used for IP at 6 µg per reaction. SPATS2 was also immunoprecipitated by rabbit anti-SPATS2.. For blotting immunoprecipitated SPATS2, NBP3-29150 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.SPATS2 AntibodyNBP3-29150] -
Whole cell lysate (10 µg) from HeLa, HEK293T, and Jurkatcells prepared using NETN lysis buffer. Antibody: Affinitypurified rabbit anti-SPATS2 antibody NBP3-29150 used for WB at 0.4 µg/ml. Detection:Chemiluminescence with an exposure time of 30 secondsApplications for SPATS2 Antibody - BSA Free
Application
Recommended Usage
Immunoprecipitation
2 - 10 µg/mg lysate
Western Blot
1:1,000 - 1:5,000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
Tris-citrate/phosphate pH 7 -8.
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: SPATS2
Alternate Names
FLJ13117, P59SCR, SCR59putative protein product of Nbla00526, Serine-rich spermatocytes and round spermatid 59 kDa protein, SPATA10serine-rich spermatocytes and round spermatid protein, spermatogenesis associated, serine-rich 2, spermatogenesis-associated serine-rich protein 2
Gene Symbol
SPATS2
Additional SPATS2 Products
Product Documents for SPATS2 Antibody - BSA Free
Certificate of Analysis
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Product Specific Notices for SPATS2 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- Immunoprecipitation Protocol
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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