Sphingomyelin Synthase 2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-60004

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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Sphingomyelin Synthase 2 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptides corresponding to SGMS2(sphingomyelin synthase 2) The peptide sequence was selected from the N terminal of SGMS2. Peptide sequence KFPLEWWKTGIAFIYAVFNLVLTTVMITVVHERVPPKELSPPLPDKFFDY. The peptide sequence for this immunogen was taken from within the described region.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Scientific Data Images for Sphingomyelin Synthase 2 Antibody - BSA Free

Western Blot: Sphingomyelin Synthase 2 Antibody [NBP1-60004]

Western Blot: Sphingomyelin Synthase 2 Antibody [NBP1-60004]

Western Blot: Sphingomyelin Synthase 2 Antibody [NBP1-60004] - Sphingomyelin Synthase 2 expression in mouse lung lysate using anti-Sphingomyelin Synthase 2 antibody. Image from verified customer review.
Western Blot: Sphingomyelin Synthase 2 Antibody [NBP1-60004]

Western Blot: Sphingomyelin Synthase 2 Antibody [NBP1-60004]

Western Blot: Sphingomyelin Synthase 2 Antibody [NBP1-60004] - Titration: 0.2-1 ug/ml, Positive Control: Human heart.

Applications for Sphingomyelin Synthase 2 Antibody - BSA Free

Application
Recommended Usage

Western Blot

1.0 ug/ml

Reviewed Applications

Read 1 review rated 4 using NBP1-60004 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 2% Sucrose

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Sphingomyelin Synthase 2

SGMS2 is a bidirectional lipid cholinephosphotransferase capable of converting phosphatidylcholine (PC) and ceramide to sphingomyelin (SM) and diacylglycerol (DAG) and vice versa. Direction is dependent on the relative concentrations of DAG and ceramide as phosphocholine acceptors. SGMS2 directly and specifically recognizes the choline head group on the substrate. SGMS2 also requires two fatty chains on the choline-P donor molecule in order to be recognized efficiently as a substrate. SGMS2 does not function strictly as a SM synthase. SGMS2 is required for cell growth.Sphingomyelin (SM) is a major component of plasma membranes. It is preferentially concentrated in the outer leaflet and has a role in the formation of lipid rafts. SM synthases (EC 2.7.8.27), such as SGMS2, produce SM in the lumen of the Golgi and on the cell surface through the transfer of phosphocholine from phosphatidylcholine onto ceramide, yielding diacylglycerol as a side product (Huitema et al., 2004 [PubMed 14685263]).[supplied by OMIM]. Sequence Note: removed 3 bases from the 5' end that did not align to the reference genome assembly. PRIMARYREFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP 1-2142 BC041369.2 4-2145

Alternate Names

EC 2.7.8.27, phosphatidylcholine:ceramide cholinephosphotransferase 2, SM synthase, sphingomyelin synthase 2SMS2MGC26963

Gene Symbol

SGMS2

UniProt

Q8NHU3 (Human)

Additional Sphingomyelin Synthase 2 Products

Product Documents for Sphingomyelin Synthase 2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Sphingomyelin Synthase 2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for Sphingomyelin Synthase 2 Antibody - BSA Free (1)

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Customer Images

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  • Name: Wen Zhang
    Application: Western Blot
    Sample Tested: mouse lung
    Species: Mouse
    Verified Customer | Posted 10/06/2014
    western blot experiment with this antibody
    Sphingomyelin Synthase 2 Antibody - BSA Free NBP1-60004

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FAQs for Sphingomyelin Synthase 2 Antibody - BSA Free

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  • Q: I am interested in your antibody anti-SMS2 cat no: NBP1-60004. Can this antibody recognize the extracellular part of SMS2 in non-permeablized cells by immunofluoresence?

    A: Thank you for contacting Novus Biologicals regarding your interest in NBP1-60004. This antibody was made against a peptide immunogen, amino acids ~70-120. According to the uniprot entry Q8NHU3, the transmembrane region begins at position 80. We have only tested this antibody for western blot, so we are unsure how it would work for ICC.

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