Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all Spi-B Primary Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human Spi-B.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Spi-B Antibody - Azide and BSA Free

Immunoprecipitation: Spi-B Antibody [NBP2-98958]

Immunoprecipitation: Spi-B Antibody [NBP2-98958]

Immunoprecipitation: Spi-B Antibody [NBP2-98958] - Spi-B was immunoprecipitated using: Lane A: 0.5 mg NIH3T3 Whole Cell Lysate 4 ul anti-Spi-B rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Spi-B rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 42 kDa. Observed band size: 44 kDa

Applications for Spi-B Antibody - Azide and BSA Free

Application
Recommended Usage

Immunoprecipitation

4-8 uL/mg of lysate

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Spi-B

SPI1 (MIM 165170) and SPIB are members of a subfamily of ETS (see ETS1; MIM 164720) transcription factors. ETS proteins share a conserved ETS domain that mediates specific DNA binding. SPIB and SPI1 bind to a purine-rich sequence, the PU box (5-prime-GAGGAA-3-prime).[supplied by OMIM]

Long Name

Transcription Factor Spi [Spleen FFV Proviral Integration Oncogene] B

Alternate Names

SpiB

Gene Symbol

SPIB

Additional Spi-B Products

Product Documents for Spi-B Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Spi-B Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

No product specific FAQs exist for this product.

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Associated Pathways

Dendritic Cell Lineage Development Pathways Dendritic Cell Lineage Development Pathway Thumbnail