Splicing Factor 1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-57322

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Splicing Factor 1 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptides corresponding to SF1(splicing factor 1) The peptide sequence was selected from the C terminal of SF1 (NP_004621). Peptide sequence MASSTPLPWQQNTTTTTTSAGTGSIPPWQQQQAAAAASPGAPQMQGNPTM. The peptide sequence for this immunogen was taken from within the described region.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

68 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Scientific Data Images for Splicing Factor 1 Antibody - BSA Free

Western Blot: Splicing Factor 1 Antibody [NBP1-57322]

Western Blot: Splicing Factor 1 Antibody [NBP1-57322]

Western Blot: Splicing Factor 1 Antibody [NBP1-57322] - Titration: 0.2-1 ug/ml, Positive Control: HepG2 cell lysate.

Applications for Splicing Factor 1 Antibody - BSA Free

Application
Recommended Usage

Western Blot

1.0 ug/ml

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 2% Sucrose

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Splicing Factor 1

SF1 contains 1 KH domain and 1 CCHC-type zinc finger. It is necessary for the ATP-dependent first step of spliceosome assembly and binds to the intron branch point sequence (BPS) 5'-UACUAAC-3' of the pre-mRNA. It may act as transcription repressor.

Alternate Names

Mammalian branch point-binding protein, mBBP, splicing factor 1, Transcription factor ZFM1, ZFM1BBP, Zinc finger gene in MEN1 locus, Zinc finger protein 162D11S636, ZNF162MBBP

Entrez Gene IDs

7536 (Human)

Gene Symbol

SF1

UniProt

Q15637 (Human)

Additional Splicing Factor 1 Products

Product Documents for Splicing Factor 1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Splicing Factor 1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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