SPRYD3 Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-77116
![Western Blot: SPRYD3 AntibodyBSA Free [NBP1-77116]](https://resources.rndsystems.com/images/products/SPRYD3-Antibody-Western-Blot-NBP1-77116-img0004.jpg "Western Blot: SPRYD3 AntibodyBSA Free [NBP1-77116]")
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Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
Antibody was raised against a 16 amino acid synthetic peptide near the center of human SPRYD3. The immunogen is located within amino acids 260 - 310 of SPRYD3. Amino Acid Squence: LARKDTPKNRHPGWSR
Specificity
SPRYD3 antibody is predicted to not cross-react with other SPRYD protein family members. At least two isoforms of SPRYD3 are known to exist.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for SPRYD3 Antibody - BSA Free
Western Blot: SPRYD3 AntibodyBSA Free [NBP1-77116]
Western Blot: SPRYD3 Antibody [NBP1-77116] - Western blot analysis of SPRYD3 in human brain tissue lysate with SPRYD3 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide![Immunohistochemistry: SPRYD3 Antibody - BSA Free [NBP1-77116]](https://resources.rndsystems.com/images/products/SPRYD3-Antibody-Immunohistochemistry-NBP1-77116-img0003.jpg "Immunohistochemistry: SPRYD3 Antibody - BSA Free [NBP1-77116]")
Immunohistochemistry: SPRYD3 Antibody - BSA Free [NBP1-77116]
Immunohistochemistry: SPRYD3 Antibody [NBP1-77116] - Immunohistochemistry of SPRYD3 in mouse brain tissue with SPRYD3 antibody at 5 ug/mL.![Immunohistochemistry: SPRYD3 Antibody - BSA Free [NBP1-77116]](https://resources.rndsystems.com/images/products/SPRYD3-Antibody-Immunohistochemistry-NBP1-77116-img0002.jpg "Immunohistochemistry: SPRYD3 Antibody - BSA Free [NBP1-77116]")
Immunohistochemistry: SPRYD3 Antibody - BSA Free [NBP1-77116]
Immunohistochemistry: SPRYD3 Antibody [NBP1-77116] - Immunofluorescence of SPRYD3 in mouse brain tissue with SPRYD3 antibody at 20 ug/mL.
Immunohistochemistry: SPRYD3 Antibody - BSA Free [NBP1-77116] -
Immunohistochemistry: SPRYD3 Antibody - BSA Free [NBP1-77116] - Immunohistochemistry of SPRYD3 in mouse brain tissue with SPRYD3 antibody at 5 u/mL.
Immunohistochemistry: SPRYD3 Antibody - BSA Free [NBP1-77116] -
Immunohistochemistry: SPRYD3 Antibody - BSA Free [NBP1-77116] - Immunohistochemistry of SPRYD3 in mouse brain tissue with SPRYD3 antibody at 5 u/mL.
Immunohistochemistry: SPRYD3 Antibody - BSA Free [NBP1-77116] -
Immunohistochemistry: SPRYD3 Antibody - BSA Free [NBP1-77116] - Immunofluorescence of SPRYD3 in mouse brain tissue with SPRYD3 antibody at 20 u/mL.
Immunohistochemistry: SPRYD3 Antibody - BSA Free [NBP1-77116] -
Immunohistochemistry: SPRYD3 Antibody - BSA Free [NBP1-77116] - Immunofluorescence of SPRYD3 in mouse brain tissue with SPRYD3 antibody at 20 u/mL.Applications for SPRYD3 Antibody - BSA Free
Application
Recommended Usage
ELISA
1:100-1:2000
Immunocytochemistry/ Immunofluorescence
20 ug/ml
Immunohistochemistry
5 ug/mL
Western Blot
1 ug/mL
Formulation, Preparation, and Storage
Purification
Peptide affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: SPRYD3
Alternate Names
FLJ14800, SPRY domain containing 3, SPRY domain-containing protein 3
Gene Symbol
SPRYD3
UniProt
Additional SPRYD3 Products
Product Documents for SPRYD3 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for SPRYD3 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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