SPRYD3 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-83681

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human, Mouse

Predicted:

Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all SPRYD3 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LARKDYPKNRHPGWSRGSVAYHADDGKIFHGSGVGDPFGPRCYKGDIMGCGIMFPRDYILDSEGDSDDSCDTVILSPTARAVRNVRNVMYLH

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SPRYD3 Antibody - BSA Free

Western Blot: SPRYD3 Antibody [NBP1-83681]

Western Blot: SPRYD3 Antibody [NBP1-83681]

Western Blot: SPRYD3 Antibody [NBP1-83681] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunocytochemistry/ Immunofluorescence: SPRYD3 Antibody [NBP1-83681]

Immunocytochemistry/ Immunofluorescence: SPRYD3 Antibody [NBP1-83681]

Immunocytochemistry/Immunofluorescence: SPRYD3 Antibody [NBP1-83681] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm, cytosol & actin filaments.
Immunohistochemistry-Paraffin: SPRYD3 Antibody [NBP1-83681]

Immunohistochemistry-Paraffin: SPRYD3 Antibody [NBP1-83681]

Immunohistochemistry-Paraffin: SPRYD3 Antibody [NBP1-83681] - Staining of human cerebral cortex.
Immunohistochemistry-Paraffin: SPRYD3 Antibody [NBP1-83681]

Immunohistochemistry-Paraffin: SPRYD3 Antibody [NBP1-83681]

Immunohistochemistry-Paraffin: SPRYD3 Antibody [NBP1-83681] - Staining of human cerebellum, cerebral cortex, kidney and lymph node using Anti-SPRYD3 antibody NBP1-83681 (A) shows similar protein distribution across tissues to independent antibody NBP2-38451 (B).
Immunohistochemistry-Paraffin: SPRYD3 Antibody [NBP1-83681]

Immunohistochemistry-Paraffin: SPRYD3 Antibody [NBP1-83681]

Immunohistochemistry-Paraffin: SPRYD3 Antibody [NBP1-83681] - Staining of human lymph node.
Immunohistochemistry-Paraffin: SPRYD3 Antibody [NBP1-83681]

Immunohistochemistry-Paraffin: SPRYD3 Antibody [NBP1-83681]

Immunohistochemistry-Paraffin: SPRYD3 Antibody [NBP1-83681] - Staining of human kidney.
Immunohistochemistry-Paraffin: SPRYD3 Antibody [NBP1-83681]

Immunohistochemistry-Paraffin: SPRYD3 Antibody [NBP1-83681]

Immunohistochemistry-Paraffin: SPRYD3 Antibody [NBP1-83681] - Staining of human cerebellum.
SPRYD3 Antibody - BSA Free Western Blot: SPRYD3 Antibody - BSA Free [NBP1-83681]

Western Blot: SPRYD3 Antibody - BSA Free [NBP1-83681]

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Human cell line RT-4
Lane 3: Human cell line U-251MG sp

Applications for SPRYD3 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SPRYD3

SPRYD3 - hypothetical protein FLJ14800

Alternate Names

FLJ14800, SPRY domain containing 3, SPRY domain-containing protein 3

Entrez Gene IDs

84926 (Human)

Gene Symbol

SPRYD3

UniProt

Q8NCJ5 (Human)

Additional SPRYD3 Products

Product Documents for SPRYD3 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for SPRYD3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for SPRYD3 Antibody - BSA Free

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  • Q: We bought for a few weeks the SPRYD3 Antibody (NBP1-83681) (100 ul). It works wonderful. We need now to use it at a known concentration. On the datasheet and the website, you indicate that the concentration is indicated on the tube. Unfortunately, when we look at it, nothing is written in front of mg/ml. Please could you let us know the concentration of the lot number : R36067.

    A: The concentration is not mentioned on the datasheet as it varies lot to lot. The concentration of Lot# R36067 is 0.1 mg/ml.

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