SRC1 Antibody (3S4C6)

Name: SRC1 Antibody (3S4C6)
Brand: Novus Biologicals
SKU: NBP3-16742
Price: 389 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-16742

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 3S4C6 expressed in HEK293

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Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 400-750 of human SRC1 (Q15788). VARSSTLPPSNSNMVSTRINRQQSSDLHSSSHSNSSNSQGSFGCSPGSQIVANVALNQGQASSQSSNPSLNLNNSPMEGTGISLAQFMSPRRQVTSGLATRPRMPNNSFPPNISTLSSPVGMTSSACNNNNRSYSNIPVTSLQGMNEGPNNSVGFSASSPVLRQMSSQNSPSRLNIQPAKAESKDNKEIASILNEMIQSDNSSSDGKPLDSGLLHNNDRLSDGDSKYSQTSHKLVQLLTTTAEQQLRHADIDTSCKDVLSCTGTSNSASANSSGGSCPSSHSSLTERHKILHRLLQEGSPSDITTLSVEPDKKDSASTSVSVTGQVQGNSSIKLELDASKKKESKDHQLLR

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SRC1 Antibody (3S4C6)

Western Blot: SRC1 Antibody (3S4C6) [NBP3-16742]

Western Blot: SRC1 Antibody (3S4C6) [NBP3-16742] - Western blot analysis of extracts of various cell lines, using SRC1 antibody (NBP3-16742) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Immunoprecipitation: SRC1 Antibody (3S4C6) [NBP3-16742]

Immunoprecipitation: SRC1 Antibody (3S4C6) [NBP3-16742] - Immunoprecipitation analysis of 300ug extracts of 293T cells using 3ug SRC1 antibody (NBP3-16742). Western blot was performed from the immunoprecipitate using SRC1 antibody (NBP3-16742) at a dilition of 1:1000.

Applications for SRC1 Antibody (3S4C6)

Application

Recommended Usage

Immunoprecipitation

1:500 - 1:1000

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SRC1

Steroid and thyroid hormones and retinoic acid regulate a complex array of gene expression activity via intracellular receptor transcription factors belonging to the ligand dependent nuclear receptor superfamily. Adding to the complexity of function of these transcription factors are associated proteins known as coactivators and corepressors which, as their names suggest, enhance or depress transcriptional activity of the nuclear receptor with which they associate. One such coactivator is Steroid Receptor Coactivator-1 (SRC 1).SRC 1 has been shown to interact with and stimulate the transcriptional activity of estrogen, progesterone, retinoic acid, thyroid and glucocorticoid receptors, as well as retinoic X receptor (RXR) in a ligand dependent manner. The amino terminal region of SRC 1 contains a PAS-A-basic helix-loop-helix homology domain which has previously been shown to be dimerization domains in other nuclear transcription factors including the aryl hydrocarbon (Ah) receptor and its heterodimerization partner, ARNT. The carboxy-terminal region of SRC 1 has been shown to possess histone acetyltransferase activity specific primarily for histones H3 & H4.

Long Name

Nuclear receptor coactivator 1

Alternate Names

bHLHe74, NCoA-1, NCOA1, Protein Hin-2, RIP160

Gene Symbol

NCOA1

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Product Documents for SRC1 Antibody (3S4C6)

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Certificate of Analysis

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Product Specific Notices for SRC1 Antibody (3S4C6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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