SRD5A2 Antibody

Novus Biologicals | Catalog # NB300-981

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Hamster IgG
View all SRD5A2 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence QVQCQQSPVLAG corresponding to N-Terminus according to NP_000339.2.

Clonality

Polyclonal

Host

Hamster

Isotype

IgG

Scientific Data Images for SRD5A2 Antibody

Western Blot: SRD5A2 Antibody [NB300-981]

Western Blot: SRD5A2 Antibody [NB300-981]

Western Blot: SRD5A2 Antibody [NB300-981] - Staining of Human Prostate lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for SRD5A2 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:8000

Western Blot

0.3-1 ug/ml
Application Notes
WB: Approx 26kDa band observed in Human Prostate and Breast lysates (calculated MW of 28.4kDa according to NP_000339.2).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SRD5A2

This gene encodes a microsomal protein expressed at high levels in androgen-sensitive tissues such as the prostate. The encoded protein is active at acidic pH and is sensitive to the 4-azasteroid inhibitor finasteride. Deficiencies in this gene can result in male pseudohermaphroditism, specifically pseudovaginal perineoscrotal hypospadias (PPSH).

Long Name

3-oxo-5-alpha-steroid 4-dehydrogenase 2

Alternate Names

5 alpha-SR2, S5AR 2, SR type 2

Entrez Gene IDs

6716 (Human)

Gene Symbol

SRD5A2

UniProt

NP_000339. (Human)

Additional SRD5A2 Products

Product Documents for SRD5A2 Antibody

Certificate of Analysis

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Product Specific Notices for SRD5A2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for SRD5A2 Antibody

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