SRD5A2 Antibody

Novus Biologicals | Catalog # NBP1-46510

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunohistochemistry-Paraffin, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all SRD5A2 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide from amino acid region 190-250 of human SRD5A2 conjugated to blue carrier protein was used as the antigen.

Reactivity Notes

Other species not yet tested but expected to work in rat.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SRD5A2 Antibody

Western Blot: SRD5A2 Antibody [NBP1-46510]

Western Blot: SRD5A2 Antibody [NBP1-46510]

Western Blot: SRD5A2 Antibody [NBP1-46510] - WB on mouse tissue lysate. Blocking: 1% LFDM for 30 min at RT; primary antibody: dilution 1:4000 incubated at 4C overnight.
Immunohistochemistry-Paraffin: SRD5A2 Antibody [NBP1-46510]

Immunohistochemistry-Paraffin: SRD5A2 Antibody [NBP1-46510]

Immunohistochemistry-Paraffin: SRD5A2 Antibody [NBP1-46510] - IHC-P on paraffin sections of human placenta. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.Detection was done using HRP polymer from manufacturers instructions; DAB chromogen.Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer.Sections were counterstained with Harris Hematoxylin.
Immunohistochemistry-Paraffin: SRD5A2 Antibody [NBP1-46510]

Immunohistochemistry-Paraffin: SRD5A2 Antibody [NBP1-46510]

Immunohistochemistry-Paraffin: SRD5A2 Antibody [NBP1-46510] - IHC-P on paraffin sections of human pancreas. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.Detection was done using using HRP polymer following manufacturers inctructions. DAB chromogen.Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer.Sections were counterstained with Harris Hematoxylin.
Immunohistochemistry-Paraffin: SRD5A2 Antibody [NBP1-46510]

Immunohistochemistry-Paraffin: SRD5A2 Antibody [NBP1-46510]

Immunohistochemistry-Paraffin: SRD5A2 Antibody [NBP1-46510] - IHC-P on paraffin sections of human pancreas. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen.Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer.Sections were counterstained with Harris Hematoxylin.
Immunohistochemistry-Paraffin: SRD5A2 Antibody [NBP1-46510]

Immunohistochemistry-Paraffin: SRD5A2 Antibody [NBP1-46510]

Immunohistochemistry-Paraffin: SRD5A2 Antibody [NBP1-46510] - IHC-P on paraffin sections of human placenta HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.
Detection was done using HRP polymer following manufacturers instructions; DAB chromogen. Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
Immunohistochemistry-Paraffin: SRD5A2 Antibody [NBP1-46510]

Immunohistochemistry-Paraffin: SRD5A2 Antibody [NBP1-46510]

Immunohistochemistry-Paraffin: SRD5A2 Antibody [NBP1-46510] - IHC-P on paraffin sections of human placenta. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.Detection was done using HRP polymer following manufacturers instructions; DAB chromogen.Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer.Sections were counterstained with Harris Hematoxylin.
Immunohistochemistry-Paraffin: SRD5A2 Antibody [NBP1-46510]

Immunohistochemistry-Paraffin: SRD5A2 Antibody [NBP1-46510]

Immunohistochemistry-Paraffin: SRD5A2 Antibody [NBP1-46510] - IHC-P on paraffin sections of human placenta. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.Detection was done using HRP polymer following manufacturers instructions; DAB chromogen.Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer.Sections were counterstained with Harris Hematoxylin.
Immunohistochemistry-Paraffin: SRD5A2 Antibody [NBP1-46510]

Immunohistochemistry-Paraffin: SRD5A2 Antibody [NBP1-46510]

Immunohistochemistry-Paraffin: SRD5A2 Antibody [NBP1-46510] - IHC-P on paraffin sections of human placenta. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.Detection was done using HRP polymer from manufacturers instructions; DAB chromogen.Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer.Sections were counterstained with Harris Hematoxylin.
Immunohistochemistry-Paraffin: SRD5A2 Antibody [NBP1-46510]

Immunohistochemistry-Paraffin: SRD5A2 Antibody [NBP1-46510]

Immunohistochemistry-Paraffin: SRD5A2 Antibody [NBP1-46510] - IHC-P on paraffin sections of human placenta. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.Detection was done using HRP polymer following manufacturers instructions; DAB chromogen.Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer.Sections were counterstained with Harris Hematoxylin.
Immunohistochemistry-Paraffin: SRD5A2 Antibody [NBP1-46510]

Immunohistochemistry-Paraffin: SRD5A2 Antibody [NBP1-46510]

Immunohistochemistry-Paraffin: SRD5A2 Antibody [NBP1-46510] - IHC-P on paraffin sections of human placenta. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.Detection was done using HRP polymer from manufacturers instructions; DAB chromogen.Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer.Sections were counterstained with Harris Hematoxylin.

Applications for SRD5A2 Antibody

Application
Recommended Usage

Immunohistochemistry

1:2000

Immunohistochemistry-Paraffin

1:2000

Western Blot

1:4000
Application Notes
The optimal dilution should be determined by the end user. Although not tested this antibody may be useful in Immunohistochemistry-Paraffin/Frozen.

Formulation, Preparation, and Storage

Purification

Unpurified

Reconstitution

Reconstitute in 0.1 ml of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability. Please note the sample size is provided in reconstituted format.

Formulation

Lyophilized from whole antisera

Preservative

No Preservative

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SRD5A2

This gene encodes a microsomal protein expressed at high levels in androgen-sensitive tissues such as the prostate. The encoded protein is active at acidic pH and is sensitive to the 4-azasteroid inhibitor finasteride. Deficiencies in this gene can result in male pseudohermaphroditism, specifically pseudovaginal perineoscrotal hypospadias (PPSH).

Long Name

3-oxo-5-alpha-steroid 4-dehydrogenase 2

Alternate Names

5 alpha-SR2, S5AR 2, SR type 2

Entrez Gene IDs

6716 (Human)

Gene Symbol

SRD5A2

Additional SRD5A2 Products

Product Documents for SRD5A2 Antibody

Certificate of Analysis

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Product Specific Notices for SRD5A2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for SRD5A2 Antibody

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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