SSB Antibody (OTI2C8)

Novus Biologicals | Catalog # NBP1-48002

Novus Biologicals

Key Product Details

Species Reactivity

Human, Canine, Monkey, Primate

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI2C8
View all SSB Primary Antibodies »

Product Specifications

Immunogen

Full-length protein expressed in 293T cell transfected with human SSB expression vector

Specificity

This antibody is specific for Homo sapiens Sjogren syndrome antigen B (autoantigen La) (SSB).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

46.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SSB Antibody (OTI2C8)

Western Blot: SSB Antibody (OTI2C8) [NBP1-48002]

Western Blot: SSB Antibody (OTI2C8) [NBP1-48002]

Western Blot: SSB Antibody (OTI2C8) [NBP1-48002] - Analysis of extracts (35ug) from 9 different cell lines by using anti-SSB monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: SSB Antibody (OTI2C8) [NBP1-48002]

Immunocytochemistry/ Immunofluorescence: SSB Antibody (OTI2C8) [NBP1-48002]

Immunocytochemistry/Immunofluorescence: SSB Antibody (OTI2C8) [NBP1-48002] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY SSB.
Immunohistochemistry-Paraffin: SSB Antibody (OTI2C8) [NBP1-48002]

Immunohistochemistry-Paraffin: SSB Antibody (OTI2C8) [NBP1-48002]

Immunohistochemistry-Paraffin: SSB Antibody (OTI2C8) [NBP1-48002] - Staining of paraffin-embedded Human prostate tissue using anti-SSB mouse monoclonal antibody.
Western Blot: SSB Antibody (OTI2C8) [NBP1-48002]

Western Blot: SSB Antibody (OTI2C8) [NBP1-48002]

Western Blot: SSB Antibody (OTI2C8) [NBP1-48002] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SSB (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SSB.
Immunohistochemistry-Paraffin: SSB Antibody (OTI2C8) [NBP1-48002]

Immunohistochemistry-Paraffin: SSB Antibody (OTI2C8) [NBP1-48002]

Immunohistochemistry-Paraffin: SSB Antibody (OTI2C8) [NBP1-48002] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-SSB mouse monoclonal antibody.
Immunohistochemistry-Paraffin: SSB Antibody (OTI2C8) [NBP1-48002]

Immunohistochemistry-Paraffin: SSB Antibody (OTI2C8) [NBP1-48002]

Immunohistochemistry-Paraffin: SSB Antibody (OTI2C8) [NBP1-48002] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-SSB mouse monoclonal antibody.
Immunohistochemistry-Paraffin: SSB Antibody (OTI2C8) [NBP1-48002]

Immunohistochemistry-Paraffin: SSB Antibody (OTI2C8) [NBP1-48002]

Immunohistochemistry-Paraffin: SSB Antibody (OTI2C8) [NBP1-48002] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-SSB mouse monoclonal antibody.
Immunohistochemistry-Paraffin: SSB Antibody (OTI2C8) [NBP1-48002]

Immunohistochemistry-Paraffin: SSB Antibody (OTI2C8) [NBP1-48002]

Immunohistochemistry-Paraffin: SSB Antibody (OTI2C8) [NBP1-48002] - Staining of paraffin-embedded Human breast tissue using anti-SSB mouse monoclonal antibody.
Immunohistochemistry-Paraffin: SSB Antibody (OTI2C8) [NBP1-48002]

Immunohistochemistry-Paraffin: SSB Antibody (OTI2C8) [NBP1-48002]

Immunohistochemistry-Paraffin: SSB Antibody (OTI2C8) [NBP1-48002] - Staining of paraffin-embedded Human colon tissue using anti-SSB mouse monoclonal antibody.
Immunohistochemistry-Paraffin: SSB Antibody (OTI2C8) [NBP1-48002]

Immunohistochemistry-Paraffin: SSB Antibody (OTI2C8) [NBP1-48002]

Immunohistochemistry-Paraffin: SSB Antibody (OTI2C8) [NBP1-48002] - Staining of paraffin-embedded Human endometrium tissue using anti-SSB mouse monoclonal antibody.
Immunohistochemistry-Paraffin: SSB Antibody (OTI2C8) [NBP1-48002]

Immunohistochemistry-Paraffin: SSB Antibody (OTI2C8) [NBP1-48002]

Immunohistochemistry-Paraffin: SSB Antibody (OTI2C8) [NBP1-48002] - Staining of paraffin-embedded Human Kidney tissue using anti-SSB mouse monoclonal antibody.
Immunohistochemistry-Paraffin: SSB Antibody (OTI2C8) [NBP1-48002]

Immunohistochemistry-Paraffin: SSB Antibody (OTI2C8) [NBP1-48002]

Immunohistochemistry-Paraffin: SSB Antibody (OTI2C8) [NBP1-48002] - Staining of paraffin-embedded Human lung tissue using anti-SSB mouse monoclonal antibody.
Immunohistochemistry-Paraffin: SSB Antibody (OTI2C8) [NBP1-48002]

Immunohistochemistry-Paraffin: SSB Antibody (OTI2C8) [NBP1-48002]

Immunohistochemistry-Paraffin: SSB Antibody (OTI2C8) [NBP1-48002] - Staining of paraffin-embedded Human Ovary tissue using anti-SSB mouse monoclonal antibody.
Immunohistochemistry-Paraffin: SSB Antibody (OTI2C8) [NBP1-48002]

Immunohistochemistry-Paraffin: SSB Antibody (OTI2C8) [NBP1-48002]

Immunohistochemistry-Paraffin: SSB Antibody (OTI2C8) [NBP1-48002] - Staining of paraffin-embedded Human pancreas tissue using anti-SSB mouse monoclonal antibody.
Immunoprecipitation: SSB Antibody (OTI2C8) [NBP1-48002]

Immunoprecipitation: SSB Antibody (OTI2C8) [NBP1-48002]

Immunoprecipitation: SSB Antibody (OTI2C8) [NBP1-48002] - (Negative control: IP without adding anti-SSB antibody.). For each experiment, 500ul of DDK tagged SSB overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-SSB antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
SSB Antibody (OTI2C8)

SSB Antibody (OTI2C8)[NBP1-48002]

SSB Antibody (OTI2C8)[NBP1-48002]Simple Western� analysis of endogenous protein TRPM4 from Jurkat lysates (0.2 mg/mL) using TRPM4 Mouse Monoclonal Antibody. The virtual lane view (left) shows the target (as indicated) at 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess� Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12�230 kDa Separation Module.

Applications for SSB Antibody (OTI2C8)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Immunoprecipitation

2ug/500ul

Western Blot

1:1000-2000
Application Notes
IP was tested using 2ug of antibody per 500ul at 0.5mg/ml overexpression lysate. Optimal dilutions for other samples will need to be determined by the end user.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.8 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SSB

La is involved in diverse aspects of RNA metabolism, including binding and protecting 3-prime UUU(H) elements of newly RNA polymerase III (see MIM 606007)-transcribed RNA, processing 5-prime and 3-prime ends of pre-tRNA precursors, acting as an RNA chaper

Alternate Names

La autoantigen, La ribonucleoprotein, lupus La protein, member 3, Sjoegren syndrome type B antigen, Sjogren syndrome antigen B (autoantigen La), SS-B, SS-B/La protein

Entrez Gene IDs

6741 (Human)

Gene Symbol

SSB

Additional SSB Products

Product Documents for SSB Antibody (OTI2C8)

Certificate of Analysis

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Product Specific Notices for SSB Antibody (OTI2C8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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